Gibberella zeae, also known by the name of its anamorph Fusarium graminearum, is a fungal plant pathogen which causes fusarium head blight (FHB), a devastating disease on wheat and barley. The pathogen is responsible for billions of dollars in economic losses worldwide each year. Infection causes shifts in the amino acid composition of wheat, resulting in shriveled kernels and contaminating the remaining grain with mycotoxins, mainly deoxynivalenol (DON), which inhibits protein biosynthesis; and zearalenone, an estrogenic mycotoxin. These toxins cause vomiting, liver damage, and reproductive defects in livestock, and are harmful to humans through contaminated food. Despite great efforts to find resistance genes against F. graminearum, no completely resistant variety is currently available. Research on the biology of F. graminearum is directed towards gaining insight into more details about the infection process and reveal weak spots in the life cycle of this pathogen to develop fungicides that can protect wheat from scab infection.
Life cycle F. graminearum is a haploid homothallic ascomycete. The fruiting bodies, perithecia, develop on the mycelium and give rise to ascospores, which land on susceptible parts of the host plant to germinate. The fungus causes fusarium head blight on wheat, barley, and other grass species, as well as ear rot on corn. The primary inocula are the ascospores, sexual spores which are produced in the perithecia. Spores are forcibly discharged and can germinate within six hours upon landing on the plant surface. The scab disease is monocyclic; after one cycle of infection with ascospores, the fungus produces macroconidia by asexual reproduction. These structures overwinter in the soil or in plant debris on the field and give rise to the mycelium in the next season.
Host and symptoms The pathogen is capable of causing a variety of diseases: head blight or 'scab' on wheat (Triticum), barley (Hordeum), rice (Oryza), oats (Avena), and Gibberella stalk and ear rot disease on maize (Zea). Additionally, the fungus may infect other plant species without causing any disease symptoms.
Corn (maize)
In Gibberella stalk rot, the leaves on early-infected plants will turn a dull greyish-green, and the lower internodes will soften and turn a tan to dark-brown. A pink-red discoloration occurs within the stalks of diseased tissue. Shredding of the pith may reveal small, round, black perithecia on the stalks. Gibberella (red) ear rot can have a reddish mold that is often at the ear tip. The infection occurs by colonizing corn silk and symptoms first occur at the ear's apex. The white mycelium turns from pink to red over time, eventually covering the entire ear. Ears that become infected early do not fully develop the reddish mold near the ear tip, as the mold grows between the husks and ear.
Rice Gibberella zeae can turn affected seeds red and cause brown discoloration in certain areas on the seed or the entire seed surface. The surface of husks develop white spots that later become yellow and salmon or carmine. Infected grains are light, shrunken and brittle. Stem nodes begin to rot and wilt, eventually causing them to turn black and disintegrate when they are infected by the fungal pathogen.
Wheat Brown, dark purple-black necrotic lesions will form on the outer surface of the spikelets, what the wheat ear breaks up into. The lesions may be referred to as scabs, but this is not to be confused and associated with other scab diseases such as those with different host and pathogen. Head blight is visible before the spikes mature. Spikelets begin to appear water-soaked before the loss of chlorophyll, which gives a white straw color. Peduncles that are directly under the inflorescence can become discolored into a brown-purple color. Tissues of the inflorescence typically become blighted into a bleached tan appearance, and the grain within it atrophies. The awn will become deformed, twisted and curve in a downward direction.
Barley Infections on barley are not always visible in the field. Similar to wheat, infected spikelets show a browning or water-soaked appearance. The infected kernels display a tan to dark brown discoloration. During long periods of wetness, pink to salmon-orange spore masses can be seen on the infected spikelets and kernels. The cortical lesions of infected seeds become a reddish-brown in cool, moist soil. Warm soil can cause head blight to occur after emergence, and crown and basal culm rot can be observed in later plant development.
Infection process
F. graminearum infects wheat spikes from anthesis through the soft dough stage of kernel development. The fungus enters the plant mostly through the flowers; however, the infection process is complex and the complete course of colonization of the host has not been described. Germ tubes seem not to be able to penetrate the hard, waxy surface of the lemma and palea which protect the flower. The fungus enters the plant through natural openings such as stomates, and needs soft tissue such as the flowers, anthers and embryo to infect the plant. From the infected floret, the fungus can grow through the rachis and cause severe damage in a short period of time under favorable conditions. Upon germination of the spores on the anthers and the surface of the developing kernel, hyphae penetrate the epicarp and spread through the seed coat. Successively, the different layers of the seed coat and finally the endosperm are colonized and killed.
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