engineering

Glycylpeptide N-tetradecanoyltransferase

Glycylpeptide N-tetradecanoyltransferase is a engineering topic covered in the lgStudy science library. This page brings together a partial reference excerpt, illustrations, worked examples, real-world applications and a short study plan, so you can understand Glycylpeptide N-tetradecanoyltransferase rather than just read about it. In short: In enzymology, a glycylpeptide N-tetradecanoyltransferase (EC 2.3.1.97) is an enzyme that catalyzes the chemical reaction tetradecanoyl-CoA + glycylpeptide ⇌ {\displaystyle \rightleftharpoons } CoA + N-tetradecanoylglycylpeptide Thus, the two substrates of this enzyme are tetradecanoyl-CoA and glycylpeptide, whereas its two products are CoA and N-tetradecanoylglycylpeptide. It participates in the N-Myristoylation of…

Key takeaways

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Reference excerpt

In enzymology, a glycylpeptide N-tetradecanoyltransferase (EC 2.3.1.97) is an enzyme that catalyzes the chemical reaction

tetradecanoyl-CoA + glycylpeptide ⇌ {\displaystyle \rightleftharpoons } CoA + N-tetradecanoylglycylpeptide Thus, the two substrates of this enzyme are tetradecanoyl-CoA and glycylpeptide, whereas its two products are CoA and N-tetradecanoylglycylpeptide. It participates in the N-Myristoylation of proteins, and in vertebrates there are two isoenzymes NMT1 and NMT2. Besides tetradecanoyl-CoA, this enzyme is also capable of using modified versions of this substrate. In human retina, an even wider range of fatty acids, including 14:1 n–9, 14:2n–6, and 12:0, are accepted by the enzyme and grafted onto guanylate cyclase activators. This is mainly a result of a special set of fatty-acid-CoA substrates available in the retina.

Nomenclature This enzyme belongs to the family of transferases, specifically those N-acyltransferases transferring groups other than aminoacyl groups (cd04301). The systematic name of this enzyme class is tetradecanoyl-CoA:glycylpeptide N-tetradecanoyltransferase. Other names in common use include peptide N-myristoyltransferase (NMT), myristoyl-CoA-protein N-myristoyltransferase, myristoyl-coenzyme A:protein N-myristoyl transferase, myristoylating enzymes, and protein N-myristoyltransferase.

Structural studies As of late 2007, 9 structures have been solved for this class of enzymes, with PDB accession codes PDB: 1IIC, PDB: 1IID, PDB: 1IYK, PDB: 1IYL, PDB: 1RXT, PDB: 2NMT, PDB: 2P6E, PDB: 2P6F, and PDB: 2P6G. The enzyme folds into two domains, each with a double EF-hand arrangement.

References

Guertin D, Grise-Miron L, Riendeau D (1986). "Identification of a 51-kilodalton polypeptide fatty acyl chain acceptor in soluble extracts from mouse cardiac tissue". Biochem. Cell Biol. 64 (12): 1249–55. Bibcode:1986BCB....64.1249G. doi:10.1139/o86-164. PMID 3566958.

Illustrations

Worked examples

Example 1 — a first encounter with Glycylpeptide N-tetradecanoyltransferase

Start with the simplest possible case. Write down what Glycylpeptide N-tetradecanoyltransferase claims or describes in one sentence, then invent the smallest concrete situation in which that sentence is true. In engineering, the smallest case is usually a single object, a single equation or a single measurement. Check that every symbol or term in your sentence has a meaning in that case.

Example 2 — changing one variable

Take the situation from Example 1 and change exactly one quantity: double it, halve it, or set it to zero. Predict what should happen to Glycylpeptide N-tetradecanoyltransferase before you calculate. Comparing your prediction with the result is the fastest way to find out whether you understand the idea or only the words.

Example 3 — an exam-style question

Typical questions about Glycylpeptide N-tetradecanoyltransferase ask you to (a) state it precisely, (b) apply it to given data, and (c) explain a limitation. Practise writing all three answers in under five minutes; the third part is what separates a full-mark answer from an average one.

Applications of Glycylpeptide N-tetradecanoyltransferase

In research: Glycylpeptide N-tetradecanoyltransferase appears in engineering research whenever the underlying quantities have to be modelled precisely. Papers usually cite it as a starting assumption and then explore where it breaks down.
In technology and industry: Engineering practice reuses Glycylpeptide N-tetradecanoyltransferase in design rules, simulations and safety margins. Knowing the idea lets you read a specification sheet and understand why the numbers look the way they do.
In the classroom: Glycylpeptide N-tetradecanoyltransferase is common in secondary-school and first-year university syllabi. It links to neighbouring topics EC 2.3.1, EC 2.3 stubs, Enzymes of known structure, so understanding it makes those chapters shorter.
In everyday life: Look for Glycylpeptide N-tetradecanoyltransferase outside the textbook — in sport, cooking, traffic, electronics or the sky above you. An example you found yourself is remembered far longer than one you were given.

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Frequently asked questions

What is Glycylpeptide N-tetradecanoyltransferase in simple terms?

In enzymology, a glycylpeptide N-tetradecanoyltransferase (EC 2.3.1.97) is an enzyme that catalyzes the chemical reaction tetradecanoyl-CoA + glycylpeptide ⇌ {\displaystyle \rightleftharpoons } CoA + N-tetradecanoylglycylpeptide Thus, the two substrates of this enzyme are tetradecanoyl-CoA and glyc…

Why does Glycylpeptide N-tetradecanoyltransferase matter?

Because it connects several engineering ideas at once: it gives you a definition you can apply, a quantity you can calculate, and a way to check whether a result is plausible.

How should I study Glycylpeptide N-tetradecanoyltransferase?

Read the excerpt, restate it from memory, then work through the examples and applications listed on this page. The five-step study plan above takes about twenty minutes.

What does this page cover?

It gives you a compact reference excerpt plus original lgStudy explanations, examples, applications and study material on Glycylpeptide N-tetradecanoyltransferase.