Gordonibacter urolithinfaciens is a Gram-positive, strictly anaerobic, non-spore-forming and motile species of bacteria in the family Eggerthellaceae. The type strain was originally isolated from the feces of a healthy human volunteer in Murcia, Spain. It can convert ellagic acid into several urolithin intermediates.
Taxonomy and nomenclature The specific epithet urolithinfaciens means "urolithin-producing", referring to the bacterium's ability to metabolize ellagic acid into urolithins. Gordonibacter faecihominis is a later heterotypic synonym of G. urolithinfaciens. Genome-based and phenotypic comparisons showed that their type strains belong to the same species.
Characteristics Cells are short rods or coccobacilli. Cells may be longitudinally asymmetrical, with one end having a conical appearance. Motility is provided by a subpolar flagellum, and some cells have been observed with a thick amorphous extracellular layer. The bacterium is catalase-positive and metabolizes a limited range of carbon sources.
Metabolism
Ellagic acid and urolithins G. urolithinfaciens converts ellagic acid sequentially into urolithin M5, urolithin M6 and urolithin C. The pathway involves opening and decarboxylation of one of the lactone rings of ellagic acid, followed by successive dehydroxylation reactions. Experiments using cell-free extracts localized the urolithin-dehydroxylating activity to the soluble cellular fraction. NADPH and flavin adenine dinucleotide participate in the electron-transfer system associated with the conversion of urolithin M5 into M6 and of M6 into urolithin C.
Other phenolic compounds In laboratory cultures, the type strain also metabolized caffeic, dihydrocaffeic, rosmarinic and chlorogenic acids through dehydroxylation or double-bond reduction. Most of the other dietary phenolic compounds tested were not transformed, indicating that these activities have a relatively restricted substrate range. In a mouse experiment, oral administration of live G. urolithinfaciens together with ellagic acid increased measured urolithin bioavailability compared with ellagic acid alone. The bacterium survived simulated gastrointestinal digestion in vitro but did not establish stable colonization in the mouse gut.
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