Gracilibacteria is a bacterial candidate phylum formerly known as GN02, BD1-5, or SN-2. It is part of the Candidate Phyla Radiation and the Patescibacteria group. The first representative of the Gracilibacteria phylum was reported in 1999 after being recovered from a deep-sea sediment sample. The representative 16S rRNA sequence was referred to as "BD1-5" (sample BD1, sequence 5) and while it was noted that it displayed low sequence identity to any known 16S rRNA gene, it was not proposed as a new phylum at this time. In 2006, representatives of Gracilibacteria were recovered from a hypersaline microbial mat from Guerrero Negro, Baja California Sur, Mexico and proposed as a new phylum "GN02". The name "Gracilibacteria" was proposed for the BD1-5/GN02 lineage in 2013, based on a substantial expansion of the genomic representation of this phylum. The first Gracilibacteria genome was recovered from an acetate-amended aquifer (Rifle, CO, USA) using culture-independent, genome-resolved metagenomic techniques in 2012. Genomic analyses suggest that members of the Gracilibacteria phylum have limited metabolisms and are likely symbionts or endosymbionts. Members of Gracilibacteria use an alternative genetic code in which UGA encodes the glycine amino acid instead of a stop codon
Phylogeny
Taxonomy The currently accepted taxonomy is based on the List of Prokaryotic names with Standing in Nomenclature (LPSN) and National Center for Biotechnology Information (NCBI). Clade "Gracilibacteria" cluster
Class "Vampirococcia" Pallen, Rodriguez-R & Alikhan 2022 (SR1; JAEDAM01) Order "Absconditibacterales" Yakimov et al. 2022 corrig. Kostovski, Oren & Göker 2025 Family "Absconditicoccaceae" Yakimov et al. 2022 ?Genus "Candidatus Strigamonas" Bouderka et al. 2024 Species "Ca. S. methylophilicida" Bouderka et al. 2024 Genus "Candidatus Absconditicoccus" Yakimov et al. 2022 Species "Ca. A. praedator" Yakimov et al. 2022 Genus "Candidatus Vampirococcus" Guerrero et al. 1986 non Kizina 2017 Species "Ca. V. lugosii" Moreira et al. 2021 Order BD1-5 Family UBA6164 Genus "Candidatus' Altimarinus" Rinke et al. 2013 Species "Ca.' A. pacificus" Rinke et al. 2013 Class "Gracilibacteriia" Yakimov et al. 2022 Order "Peribacterales" Anantharaman et al. 2016 Family "Peribacteraceae" Anantharaman et al. 2016 Genus "Candidatus Peribacter" Anantharaman et al. 2016 Species "Ca. P. riflensis" Anantharaman et al. 2016
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