Haloarcula hispanica SH1 virus is a double-stranded DNA virus that infects the archaeon Haloarcula hispanica.
Virology There are 15 structural proteins in the capsid varying in molecular weight between 185 kilodaltons (kDa) and 4 kDa. These have been numbered from VP1 to VP 15. Seven appear to be minor components (VP5, VP6, VP8, VP11, VP13, VP14 and VP15). VP12 (molecular weight 9.8 kDa) has two membrane spanning helices suggesting it is an integral membrane protein. VP2 may be an elongated fiber like protein suitable for forming a spike. The lipid membrane contains phosphatidylglycerol, phosphatidylglycerophosphate methyl ester and phosphatidylglycerosulfate.
Genome The genome is linear 30,898 base pairs in length and contains 309 base pair inverted terminal repeat sequences. The G+C content is 68.4%. It encodes 56 open reading frames (ORFs). The structural proteins correspond to the ORFs as follows:
ORF13 – VP1 ORF23 – VP12 ORF24 – VP7 ORF25 – VP4 ORF27 – VP13 ORF28 – VP2 ORF29 – VP5 ORF30 – VP10 ORF31 – VP9 ORF32 – VP3 ORF33 – VP6 All the structural proteins are located in the middle of the genome. ORF 17 encodes a protein with an ATPase domain.
References
