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Hydrogenobyrinic acid a,c-diamide synthase (glutamine-hydrolysing)

Hydrogenobyrinic acid a,c-diamide synthase (glutamine-hydrolysing) is a chemistry topic covered in the lgStudy science library. This page brings together a partial reference excerpt, illustrations, worked examples, real-world applications and a short study plan, so you can understand Hydrogenobyrinic acid a,c-diamide synthase (glutamine-hydrolysing) rather than just read about it. In short: In enzymology, a hydrogenobyrinic acid a,c-diamide synthase (glutamine-hydrolysing) (EC 6.3.5.9) is an enzyme that catalyzes the chemical reaction 2 ATP + hydrogenobyrinic acid + 2 L-glutamine + 2 H2O ⇌ {\displaystyle \rightleftharpoons } 2 ADP + 2 phosphate + hydrogenobyrinic acid a,c-diamide + 2 L-glutamate The four substrates of this enzyme are ATP, hydrogenobyrinic acid, L-glutamine, and H2O; its four products a…

Key takeaways

  • Hydrogenobyrinic acid a,c-diamide synthase (glutamine-hydrolysing) belongs to chemistry; place it in that map before memorising details.
  • Learn the definition first, then one example that makes the definition concrete.
  • Connect Hydrogenobyrinic acid a,c-diamide synthase (glutamine-hydrolysing) to a quantity you can measure, compute or draw — that is where exam questions come from.
  • Reproduce the core statement of Hydrogenobyrinic acid a,c-diamide synthase (glutamine-hydrolysing) from memory before moving on to harder problems.

Reference excerpt

In enzymology, a hydrogenobyrinic acid a,c-diamide synthase (glutamine-hydrolysing) (EC 6.3.5.9) is an enzyme that catalyzes the chemical reaction

2 ATP + hydrogenobyrinic acid + 2 L-glutamine + 2 H2O ⇌ {\displaystyle \rightleftharpoons } 2 ADP + 2 phosphate + hydrogenobyrinic acid a,c-diamide + 2 L-glutamate The four substrates of this enzyme are ATP, hydrogenobyrinic acid, L-glutamine, and H2O; its four products are ADP, phosphate, hydrogenobyrinic acid a,c-diamide, and L-glutamate. This enzyme belongs to the family of ligases, specifically those forming carbon-nitrogen bonds carbon-nitrogen ligases with glutamine as amido-N-donor. The systematic name of this enzyme class is hydrogenobyrinic-acid:L-glutamine amido-ligase (AMP-forming). This enzyme is also called CobB and is part of the biosynthetic pathway to cobalamin (vitamin B12) in aerobic bacteria.

See also Cobalamin biosynthesis

References

Debussche L, Thibaut D, Cameron B, Crouzet J, Blanche F (1990). "Purification and characterization of cobyrinic acid a,c-diamide synthase from Pseudomonas denitrificans". J. Bacteriol. 172 (11): 6239–44. doi:10.1128/jb.172.11.6239-6244.1990. PMC 526805. PMID 2172209. Warren MJ, Raux E, Schubert HL, Escalante-Semerena JC (2002). "The biosynthesis of adenosylcobalamin (vitamin B12)". Nat. Prod. Rep. 19 (4): 390–412. doi:10.1039/b108967f. PMID 12195810.

Worked examples

Example 1 — a first encounter with Hydrogenobyrinic acid a,c-diamide synthase (glutamine-hydrolysing)

Start with the simplest possible case. Write down what Hydrogenobyrinic acid a,c-diamide synthase (glutamine-hydrolysing) claims or describes in one sentence, then invent the smallest concrete situation in which that sentence is true. In chemistry, the smallest case is usually a single object, a single equation or a single measurement. Check that every symbol or term in your sentence has a meaning in that case.

Example 2 — changing one variable

Take the situation from Example 1 and change exactly one quantity: double it, halve it, or set it to zero. Predict what should happen to Hydrogenobyrinic acid a,c-diamide synthase (glutamine-hydrolysing) before you calculate. Comparing your prediction with the result is the fastest way to find out whether you understand the idea or only the words.

Example 3 — an exam-style question

Typical questions about Hydrogenobyrinic acid a,c-diamide synthase (glutamine-hydrolysing) ask you to (a) state it precisely, (b) apply it to given data, and (c) explain a limitation. Practise writing all three answers in under five minutes; the third part is what separates a full-mark answer from an average one.

Applications of Hydrogenobyrinic acid a,c-diamide synthase (glutamine-hydrolysing)

In research
Hydrogenobyrinic acid a,c-diamide synthase (glutamine-hydrolysing) appears in chemistry research whenever the underlying quantities have to be modelled precisely. Papers usually cite it as a starting assumption and then explore where it breaks down.
In technology and industry
Engineering practice reuses Hydrogenobyrinic acid a,c-diamide synthase (glutamine-hydrolysing) in design rules, simulations and safety margins. Knowing the idea lets you read a specification sheet and understand why the numbers look the way they do.
In the classroom
Hydrogenobyrinic acid a,c-diamide synthase (glutamine-hydrolysing) is common in secondary-school and first-year university syllabi. It links to neighbouring topics EC 6.3.5, Enzymes of unknown structure, Ligase stubs, so understanding it makes those chapters shorter.
In everyday life
Look for Hydrogenobyrinic acid a,c-diamide synthase (glutamine-hydrolysing) outside the textbook — in sport, cooking, traffic, electronics or the sky above you. An example you found yourself is remembered far longer than one you were given.
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How to study Hydrogenobyrinic acid a,c-diamide synthase (glutamine-hydrolysing) in 20 minutes

  1. Read the reference excerpt below once, without taking notes.
  2. Close the page and write down what Hydrogenobyrinic acid a,c-diamide synthase (glutamine-hydrolysing) means in your own words.
  3. Compare your version with the excerpt and mark what you missed.
  4. Work through the three examples above with pen and paper.
  5. Explain Hydrogenobyrinic acid a,c-diamide synthase (glutamine-hydrolysing) out loud to somebody else — or to Teacher Smith in the lgStudy chat.

Frequently asked questions

What is Hydrogenobyrinic acid a,c-diamide synthase (glutamine-hydrolysing) in simple terms?

In enzymology, a hydrogenobyrinic acid a,c-diamide synthase (glutamine-hydrolysing) (EC 6.3.5.9) is an enzyme that catalyzes the chemical reaction 2 ATP + hydrogenobyrinic acid + 2 L-glutamine + 2 H2O ⇌ {\displaystyle \rightleftharpoons } 2 ADP + 2 phosphate + hydrogenobyrinic acid a,c-diamide + 2…

Why does Hydrogenobyrinic acid a,c-diamide synthase (glutamine-hydrolysing) matter?

Because it connects several chemistry ideas at once: it gives you a definition you can apply, a quantity you can calculate, and a way to check whether a result is plausible.

How should I study Hydrogenobyrinic acid a,c-diamide synthase (glutamine-hydrolysing)?

Read the excerpt, restate it from memory, then work through the examples and applications listed on this page. The five-step study plan above takes about twenty minutes.

What does this page cover?

It gives you a compact reference excerpt plus original lgStudy explanations, examples, applications and study material on Hydrogenobyrinic acid a,c-diamide synthase (glutamine-hydrolysing).

Tags

  • EC 6.3.5
  • Enzymes of unknown structure
  • Ligase stubs

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