In cell biology, extracellular fluid (ECF) denotes all body fluid outside the cells of any multicellular organism. Total body water in healthy adults is about 50–60% (range 45 to 75%) of total body weight; women and the obese typically have a lower percentage than lean men. Extracellular fluid makes up about one-third of body fluid, the remaining two-thirds is intracellular fluid within cells. The main component of the extracellular fluid is the interstitial fluid that surrounds cells. Extracellular fluid is the internal environment of all multicellular animals, and in those animals with a blood circulatory system, a proportion of this fluid is blood plasma. Plasma and interstitial fluid are the two components that make up at least 97% of the ECF. Lymph makes up a small percentage of the interstitial fluid. The remaining small portion of the ECF includes the transcellular fluid (about 2.5%). The ECF can also be seen as having two components – plasma and lymph as a delivery system, and interstitial fluid for water and solute exchange with the cells. The extracellular fluid, in particular the interstitial fluid, constitutes the body's internal environment that bathes all of the cells in the body. The ECF composition is therefore crucial for their normal functions, and is maintained by a number of homeostatic mechanisms involving negative feedback. Homeostasis regulates, among others, the pH, sodium, potassium, and calcium concentrations in the ECF. The volume of body fluid, blood glucose, oxygen, and carbon dioxide levels are also tightly homeostatically maintained. The volume of extracellular fluid in a young adult male of 70 kg (154 lbs) is 20% of body weight – about fourteen liters. Eleven liters are interstitial fluid and the remaining three liters are plasma.
Components The main component of the extracellular fluid (ECF) is the interstitial fluid, or tissue fluid, which surrounds the cells in the body. The other major component of the ECF is the intravascular fluid of the circulatory system called blood plasma. The remaining small percentage of ECF includes the transcellular fluid. These constituents are often called "fluid compartments". The volume of extracellular fluid in a young adult male of 70 kg, is 20% of body weight – about fourteen liters.
Interstitial fluid
Interstitial fluid is essentially comparable to plasma. The interstitial fluid and plasma make up about 97% of the ECF, and a small percentage of this is lymph. Interstitial fluid is the body fluid between blood vessels and cells, containing nutrients from capillaries by diffusion and holding waste products discharged by cells due to metabolism. 11 liters of the ECF are interstitial fluid and the remaining three liters are plasma. Plasma and interstitial fluid are very similar because water, ions, and small solutes are continuously exchanged between them across the walls of capillaries, through pores and capillary clefts. Interstitial fluid consists of a water solvent containing sugars, salts, fatty acids, amino acids, coenzymes, hormones, neurotransmitters, white blood cells and cell waste-products. This solution accounts for 26% of the water in the human body. The composition of interstitial fluid depends upon the exchanges between the cells in the biological tissue and the blood. This means that tissue fluid has a different composition in different tissues and in different areas of the body. The plasma that filters through the blood capillaries into the interstitial fluid does not contain red blood cells or platelets as they are too large to pass through but can contain some white blood cells to help the immune system. Once the extracellular fluid collects into small vessels (lymph capillaries) it is considered to be lymph, and the vessels that carry it back to the blood are called the lymphatic vessels. The lymphatic system returns protein and excess interstitial fluid to the circulation. The ionic composition of the interstitial fluid and blood plasma vary due to the Gibbs–Donnan effect. This causes a slight difference in the concentration of cations and anions between the two fluid compartments.
Transcellular fluid
Transcellular fluid is formed from the transport activities of cells, and is the smallest component of extracellular fluid. These fluids are contained within epithelial lined spaces. Examples of this fluid are cerebrospinal fluid, aqueous humor in the eye, serous fluid in the serous membranes lining body cavities, perilymph and endolymph in the inner ear, and joint fluid. Due to the varying locations of transcellular fluid, the composition changes dramatically. Some of the electrolytes present in the transcellular fluid are sodium ions, chloride ions, and bicarbonate ions.
Function
Extracellular fluid provides the medium for the exchange of substances between the ECF and the cells, and this can take place through dissolving, mixing and transporting in the fluid medium. Substances in the ECF include dissolved gases, nutrients, and electrolytes, all needed to maintain life. ECF also contains materials secreted from cells in soluble form, but which quickly coalesce into fibers (e.g. collagen, reticular, and elastic fibres) or precipitates out into a solid or semisolid form (e.g. proteoglycans which form the bulk of cartilage, and the components of bone). These and many other substances occur, especially in association with various proteoglycans, to form the extracellular matrix, or the "filler" substance, between the cells throughout the body. These substances occur in the extracellular space, and are therefore all bathed or soaked in ECF, without being part of it.
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