Lactobacillus paragasseri is a species of lactic acid bacterium in the family Lactobacillaceae. It is closely related to Lactobacillus gasseri. Its cells are Gram-positive, rod-shaped, non-motile, non-spore-forming, and catalase-negative. The type strain is facultatively anaerobic and was isolated from human feces.
Taxonomy The species was delineated through a genome-based reassessment of strains JCM 5343T, JCM 5344, and JCM 1130, which had previously been identified as L. gasseri. A later genomic survey found that the full-length 16S rRNA gene sequences of L. paragasseri and L. gasseri differ by only two nucleotides. Twenty publicly deposited genomes labelled as L. gasseri were reassigned to L. paragasseri using whole-genome comparisons. Whole-genome data are therefore generally needed to distinguish the two species reliably. The specific epithet combines the Greek prefix para-, meaning resembling, with gasseri, the epithet of L. gasseri; the name therefore means “resembling Lactobacillus gasseri”.
Characteristics The strains used to delimit the species grow at temperatures from 25 to 45 °C and in medium containing 2.0% sodium chloride. Their major cellular fatty acids are C16:0 and C18:1 ω9c. The type strain forms circular, grey-white colonies on MRS agar.
Isolation sources Genome metadata and associated literature have recorded L. paragasseri isolates from human stool and other gastrointestinal samples, the vaginal tract, urine, lung or aspirate samples, breast milk, a wound, and the oral cavity. The type strain, JCM 5343T, was isolated from human feces, while strain JCM 5344 was isolated from the vaginal tract. Genome-sequenced strains have also been cultured from catheterized and voided urine. Three strains were recovered from voided urine samples from women with type 2 diabetes.
Genomic and biochemical studies In a comparative analysis of 79 publicly available L. gasseri and L. paragasseri genome assemblies, every L. paragasseri genome examined contained genes for one or both of the bacteriocins gassericin T and gassericin S. In vitro characterization of the type strain identified a bile salt hydrolase that hydrolyzed conjugated bile salts and also showed activity against penicillin.
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