Limonin-D-ring-lactonase (EC 3.1.1.36) is an enzyme that catalyzes the reaction
The enzyme characterised from Citrus seeds hydrolyses the triterpenoid, limonin, specifically at the lactone forming the D-ring. The product, limonoate A-ring-lactone, retains its A-ring lactone. Limonin is a bitter-tasting component of the seeds but limonoate A-ring-lactone is less bitter. This enzyme is a hydrolase, specifically one acting on carboxylic ester bonds. The systematic name of this enzyme class is limonoate-D-ring-lactone lactonohydrolase. Other names in common use include limonin-D-ring-lactone hydrolase, and limonin lactone hydrolase.
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