This list contains a list of EC numbers for the third group, EC 3, hydrolases, placed in numerical order as determined by the Nomenclature Committee of the International Union of Biochemistry and Molecular Biology. All official information is tabulated at the website of the committee. The database is developed and maintained by Andrew McDonald.
EC 3.1: Acting on Ester Bonds
EC 3.1.1: Carboxylic Ester Hydrolases EC 3.1.1.1: carboxylesterase EC 3.1.1.2: arylesterase EC 3.1.1.3: triacylglycerol lipase EC 3.1.1.4: phospholipase A2 EC 3.1.1.5: lysophospholipase EC 3.1.1.6: acetylesterase EC 3.1.1.7: acetylcholinesterase EC 3.1.1.8: cholinesterase EC 3.1.1.9: deleted, a side reaction of EC 3.1.1.8 cholinesterase EC 3.1.1.10: tropinesterase EC 3.1.1.11: pectinesterase EC 3.1.1.12: deleted, identical with EC 3.1.1.1 carboxylesterase EC 3.1.1.13: sterol esterase EC 3.1.1.14: chlorophyllase EC 3.1.1.15: L-arabinonolactonase EC 3.1.1.16: deleted, mixture of EC 5.3.3.4 (muconolactone Δ-isomerase) and EC 3.1.1.24 (3-oxoadipate enol-lactonase) EC 3.1.1.17: gluconolactonase EC 3.1.1.18: deleted, now included with EC 3.1.1.17 gluconolactonase EC 3.1.1.19: uronolactonase EC 3.1.1.20: tannase EC 3.1.1.21: deleted, now known to be catalysed by EC 3.1.1.1, carboxylesterase and EC 3.1.1.3, triacylglycerol lipase. EC 3.1.1.22: hydroxybutyrate-dimer hydrolase EC 3.1.1.23: acylglycerol lipase EC 3.1.1.24: 3-oxoadipate enol-lactonase EC 3.1.1.25: 1,4-lactonase EC 3.1.1.26: galactolipase EC 3.1.1.27: 4-pyridoxolactonase EC 3.1.1.28: acylcarnitine hydrolase EC 3.1.1.29: aminoacyl-tRNA hydrolase EC 3.1.1.30: D-arabinonolactonase EC 3.1.1.31: 6-phosphogluconolactonase EC 3.1.1.32: phospholipase A1 EC 3.1.1.33: 6-acetylglucose deacetylase EC 3.1.1.34: lipoprotein lipase EC 3.1.1.35: dihydrocoumarin hydrolase EC 3.1.1.36: limonin-D-ring-lactonase EC 3.1.1.37: steroid-lactonase EC 3.1.1.38: triacetate-lactonase EC 3.1.1.39: actinomycin lactonase EC 3.1.1.40: orsellinate-depside hydrolase EC 3.1.1.41: cephalosporin-C deacetylase EC 3.1.1.42: chlorogenate hydrolase EC 3.1.1.43: α-amino-acid esterase EC 3.1.1.44: 4-methyloxaloacetate esterase EC 3.1.1.45: carboxymethylenebutenolidase EC 3.1.1.46: deoxylimonate A-ring-lactonase EC 3.1.1.47: 1-alkyl-2-acetylglycerophosphocholine esterase EC 3.1.1.48: fusarinine-C ornithinesterase EC 3.1.1.49: sinapine esterase EC 3.1.1.50: wax-ester hydrolase EC 3.1.1.51: phorbol-diester hydrolase EC 3.1.1.52: phosphatidylinositol deacylase EC 3.1.1.53: sialate O-acetylesterase EC 3.1.1.54: acetoxybutynylbithiophene deacetylase EC 3.1.1.55: acetylsalicylate deacetylase EC 3.1.1.56: methylumbelliferyl-acetate deacetylase EC 3.1.1.57: 2-pyrone-4,6-dicarboxylate lactonase EC 3.1.1.58: N-acetylgalactosaminoglycan deacetylase EC 3.1.1.59: juvenile-hormone esterase EC 3.1.1.60: bis(2-ethylhexyl)phthalate esterase EC 3.1.1.61: protein-glutamate methylesterase EC 3.1.1.62: Now listed as EC 3.5.1.47, N-acetyldiaminopimelate deacetylase EC 3.1.1.63: 11-cis-retinyl-palmitate hydrolase EC 3.1.1.64: retinoid isomerohydrolase EC 3.1.1.65: L-rhamnono-1,4-lactonase EC 3.1.1.66: 5-(3,4-diacetoxybut-1-ynyl)-2,2′-bithiophene deacetylase EC 3.1.1.67: fatty-acyl-ethyl-ester synthase EC 3.1.1.68: xylono-1,4-lactonase EC 3.1.1.69: Now EC 3.5.1.89, N-acetylglucosaminylphosphatidylinositol deacetylase EC 3.1.1.70: cetraxate benzylesterase EC 3.1.1.71: acetylalkylglycerol acetylhydrolase EC 3.1.1.72: acetylxylan esterase EC 3.1.1.73: feruloyl esterase EC 3.1.1.74: cutinase EC 3.1.1.75: poly(3-hydroxybutyrate) depolymerase EC 3.1.1.76: poly(3-hydroxyoctanoate) depolymerase EC 3.1.1.77: acyloxyacyl hydrolase EC 3.1.1.78: polyneuridine-aldehyde esterase EC 3.1.1.79: hormone-sensitive lipase EC 3.1.1.80: acetylajmaline esterase EC 3.1.1.81: quorum-quenching N-acyl-homoserine lactonase EC 3.1.1.82: pheophorbidase EC 3.1.1.83: monoterpene ε-lactone hydrolase EC 3.1.1.84: cocaine esterase EC 3.1.1.85: pimelyl-(acyl-carrier protein) methyl ester esterase EC 3.1.1.86: rhamnogalacturonan acetylesterase EC 3.1.1.87: fumonisin B1 esterase EC 3.1.1.88: pyrethroid hydrolase EC 3.1.1.89: protein phosphatase methylesterase-1 EC 3.1.1.90: all-trans-retinyl ester 13-cis isomerohydrolase EC 3.1.1.91: 2-oxo-3-(5-oxofuran-2-ylidene)propanoate lactonase EC 3.1.1.92: 4-sulfomuconolactone hydrolase EC 3.1.1.93: mycophenolic acid acyl-glucuronide esterase EC 3.1.1.94: versiconal hemiacetal acetate esterase EC 3.1.1.95: aclacinomycin methylesterase EC 3.1.1.96: D-aminoacyl-tRNA deacylase EC 3.1.1.97: methylated diphthine methylhydrolase EC 3.1.1.98: [Wnt protein] O-palmitoleoyl-L-serine hydrolase EC 3.1.1.99: 6-deoxy-6-sulfogluconolactonase EC 3.1.1.100: chlorophyllide a hydrolase EC 3.1.1.101: poly(ethylene terephthalate) hydrolase EC 3.1.1.102: mono(ethylene terephthalate) hydrolase EC 3.1.1.103: teichoic acid D-alanine hydrolase EC 3.1.1.104: 5-phospho-D-xylono-1,4-lactonase EC 3.1.1.105: 3-O-acetylpapaveroxine carboxylesterase EC 3.1.1.106: O-acetyl-ADP-ribose deacetylase EC 3.1.1.107: apo-salmochelin esterase EC 3.1.1.108: iron(III)-enterobactin esterase EC 3.1.1.109: iron(III)-salmochelin esterase EC 3.1.1.110: xylono-1,5-lactonase EC 3.1.1.111: phosphatidylserine sn-1 acylhydrolase EC 3.1.1.112: isoamyl acetate esterase EC 3.1.1.113: ethyl acetate hydrolase EC 3.1.1.114: methyl acetate hydrolase EC 3.1.1.115: D-apionolactonase EC 3.1.1.116: sn-1-specific diacylglycerol lipase EC 3.1.1.117: (4-O-methyl)-D-glucuronate—lignin esterase EC 3.1.1.118: phospholipid sn-1 acylhydrolase
EC 3.1.2: Thioester Hydrolases EC 3.1.2.1: acetyl-CoA hydrolase EC 3.1.2.2: palmitoyl-CoA hydrolase EC 3.1.2.3: succinyl-CoA hydrolase EC 3.1.2.4: 3-hydroxyisobutyryl-CoA hydrolase EC 3.1.2.5: hydroxymethylglutaryl-CoA hydrolase EC 3.1.2.6: hydroxyacylglutathione hydrolase EC 3.1.2.7: glutathione thiolesterase EC 3.1.2.8: Now included with EC 3.1.2.6 hydroxyacylglutathione hydrolase EC 3.1.2.9: S-acetoacetylhydrolipoate hydrolase deleted EC 3.1.2.10: formyl-CoA hydrolase EC 3.1.2.11: acetoacetyl-CoA hydrolase EC 3.1.2.12: S-formylglutathione hydrolase EC 3.1.2.13: S-succinylglutathione hydrolase EC 3.1.2.14: oleoyl-[acyl-carrier-protein] hydrolase EC 3.1.2.15: covered by EC 3.4.19.12, ubiquitinyl hydrolase 1 EC 3.1.2.16: citrate lyase deacetylase EC 3.1.2.17: (S)-methylmalonyl-CoA hydrolase EC 3.1.2.18: ADP-dependent short-chain-acyl-CoA hydrolase EC 3.1.2.19: ADP-dependent medium-chain-acyl-CoA hydrolase EC 3.1.2.20: acyl-CoA hydrolase EC 3.1.2.21: dodecanoyl-(acyl-carrier-protein) hydrolase EC 3.1.2.22: palmitoyl[protein] hydrolase EC 3.1.2.23: 4-hydroxybenzoyl-CoA thioesterase EC 3.1.2.24: transferred entry now EC 3.13.1.3, 2′-hydroxybiphenyl-2-sulfinate desulfinase. EC 3.1.2.25: phenylacetyl-CoA hydrolase EC 3.1.2.26: Now EC 2.8.3.25, bile acid CoA transferase EC 3.1.2.27: choloyl-CoA hydrolase EC 3.1.2.28: 1,4-dihydroxy-2-naphthoyl-CoA hydrolase EC 3.1.2.29: fluoroacetyl-CoA thioesterase EC 3.1.2.30: (3S)-malyl-CoA thioesterase EC 3.1.2.31: dihydromonacolin L-[lovastatin nonaketide synthase] thioesterase EC 3.1.2.32: 2-aminobenzoylacetyl-CoA thioesterase
EC 3.1.3: Phosphoric Monoester Hydrolases EC 3.1.3.1: alkaline phosphatase EC 3.1.3.2: acid phosphatase EC 3.1.3.3: phosphoserine phosphatase EC 3.1.3.4: phosphatidate phosphatase EC 3.1.3.5: 5′-nucleotidase EC 3.1.3.6: 3′-nucleotidase EC 3.1.3.7: 3′(2′),5′-bisphosphate nucleotidase EC 3.1.3.8: 3-phytase EC 3.1.3.9: glucose-6-phosphatase EC 3.1.3.10: glucose-1-phosphatase EC 3.1.3.11: fructose-bisphosphatase EC 3.1.3.12: trehalose-phosphatase EC 3.1.3.13: Recent studies have shown that this is a partial activity of EC 5.4.2.11, phosphoglycerate mutase (2,3-diphosphoglycerate-dependent) EC 3.1.3.14: methylphosphothioglycerate phosphatase EC 3.1.3.15: histidinol-phosphatase EC 3.1.3.16: protein serine/threonine phosphatase EC 3.1.3.17: (phosphorylase) phosphatase EC 3.1.3.18: phosphoglycolate phosphatase EC 3.1.3.19: glycerol-2-phosphatase EC 3.1.3.20: phosphoglycerate phosphatase EC 3.1.3.21: glycerol-1-phosphatase EC 3.1.3.22: mannitol-1-phosphatase EC 3.1.3.23: sugar-phosphatase EC 3.1.3.24: sucrose-phosphatase EC 3.1.3.25: inositol-phosphate phosphatase EC 3.1.3.26: 4-phytase EC 3.1.3.27: phosphatidylglycerophosphatase EC 3.1.3.28: ADP-phosphoglycerate phosphatase EC 3.1.3.29: N-acylneuraminate-9-phosphatase EC 3.1.3.30: The activity may be that of an acid phosphatase EC 3.1.3.31: The activity may be that of an acid phosphatase EC 3.1.3.32: polynucleotide 3′-phosphatase EC 3.1.3.33: polynucleotide 5′-phosphatase EC 3.1.3.34: deoxynucleotide 3′-phosphatase EC 3.1.3.35: thymidylate 5′-phosphatase EC 3.1.3.36: phosphoinositide 5-phosphatase EC 3.1.3.37: sedoheptulose-bisphosphatase EC 3.1.3.38: 3-phosphoglycerate phosphatase EC 3.1.3.39: streptomycin-6-phosphatase EC 3.1.3.40: guanidinodeoxy-scyllo-inositol-4-phosphatase EC 3.1.3.41: 4-nitrophenylphosphatase EC 3.1.3.42: [glycogen-synthase-D] phosphatase EC 3.1.3.43: [pyruvate dehydrogenase (acetyl-transferring)]-phosphatase EC 3.1.3.44: [acetyl-CoA carboxylase]-phosphatase EC 3.1.3.45: 3-deoxy-manno-octulosonate-8-phosphatase EC 3.1.3.46: fructose-2,6-bisphosphate 2-phosphatase EC 3.1.3.47: [hydroxymethylglutaryl-CoA reductase (NADPH)]-phosphatase EC 3.1.3.48: protein-tyrosine-phosphatase EC 3.1.3.49: [pyruvate kinase]-phosphatase EC 3.1.3.50: sorbitol-6-phosphatase EC 3.1.3.51: dolichyl-phosphatase EC 3.1.3.52: [3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring)]-phosphatase EC 3.1.3.53: [myosin-light-chain] phosphatase EC 3.1.3.54: fructose-2,6-bisphosphate 6-phosphatase EC 3.1.3.55: caldesmon-phosphatase EC 3.1.3.56: inositol-polyphosphate 5-phosphatase EC 3.1.3.57: inositol-1,4-bisphosphate 1-phosphatase EC 3.1.3.58: sugar-terminal-phosphatase EC 3.1.3.59: alkylacetylglycerophosphatase EC 3.1.3.60: phosphoenolpyruvate phosphatase EC 3.1.3.61: deleted, as its existence has not been established EC 3.1.3.62: multiple inositol-polyphosphate phosphatase EC 3.1.3.63: 2-carboxy-D-arabinitol-1-phosphatase EC 3.1.3.64: phosphatidylinositol-3-phosphatase EC 3.1.3.65: Now included with EC 3.1.3.64, phosphatidylinositol-3-phosphatase EC 3.1.3.66: phosphatidylinositol-3,4-bisphosphate 4-phosphatase EC 3.1.3.67: phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase EC 3.1.3.68: 2-deoxyglucose-6-phosphatase EC 3.1.3.69: glucosylglycerol 3-phosphatase EC 3.1.3.70: mannosyl-3-phosphoglycerate phosphatase EC 3.1.3.71: 2-phosphosulfolactate phosphatase EC 3.1.3.72: 5-phytase EC 3.1.3.73: adenosylcobalamin/α-ribazole phosphatase EC 3.1.3.74: pyridoxal phosphatase EC 3.1.3.75: phosphoethanolamine/phosphocholine phosphatase EC 3.1.3.76: lipid-phosphate phosphatase EC 3.1.3.77: acireductone synthase EC 3.1.3.78: phosphatidylinositol-4,5-bisphosphate 4-phosphatase EC 3.1.3.79: mannosylfructose-phosphate phosphatase EC 3.1.3.80: 2,3-bisphosphoglycerate 3-phosphatase EC 3.1.3.81: Transferred entry, now EC 3.6.1.75, diacylglycerol diphosphate phosphatase EC 3.1.3.82: D-glycero-β-D-manno-heptose 1,7-bisphosphate 7-phosphatase EC 3.1.3.83: D-glycero-α-D-manno-heptose 1,7-bisphosphate 7-phosphatase EC 3.1.3.84: ADP-ribose 1″-phosphate phosphatase EC 3.1.3.85: glucosyl-3-phosphoglycerate phosphatase EC 3.1.3.86: phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase EC 3.1.3.87: 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase EC 3.1.3.88: 5′′-phosphoribostamycin phosphatase
EC 3.1.4: Phosphoric Diester Hydrolases EC 3.1.4.1: phosphodiesterase I EC 3.1.4.2: glycerophosphocholine phosphodiesterase EC 3.1.4.3: lecithinase C EC 3.1.4.4: phospholipase D EC 3.1.4.5: Now EC 3.1.21.1, deoxyribonuclease I EC 3.1.4.6: Now EC 3.1.22.1, deoxyribonuclease II EC 3.1.4.7: Now EC 3.1.31.1, micrococcal nuclease EC 3.1.4.8: Now EC 3.1.27.3, ribonuclease T1 EC 3.1.4.9: Now EC 3.1.30.2, Serratia marcescens nuclease EC 3.1.4.10: Now EC 4.6.1.13, phosphatidylinositol diacylglycerol-lyase EC 3.1.4.11: phosphoinositide phospholipase C EC 3.1.4.12: sphingomyelin phosphodiesterase EC 3.1.4.13: serine-ethanolaminephosphate phosphodiesterase EC 3.1.4.14: [acyl-carrier-protein] phosphodiesterase EC 3.1.4.15 : transferred to EC 2.7.7.89, adenylyl-[glutamateammonia ligase] phosphorylase EC 3.1.4.16: 2′,3′-cyclic-nucleotide 2′-phosphodiesterase EC 3.1.4.17: 3′,5′-cyclic-nucleotide phosphodiesterase EC 3.1.4.18: Now EC 3.1.16.1, spleen exonuclease EC 3.1.4.19: Now EC 3.1.13.3, oligonucleotidase EC 3.1.4.20: Now EC 3.1.13.1, exoribonuclease II EC 3.1.4.21: Now EC 3.1.30.1, Aspergillus nuclease S1 EC 3.1.4.22: Now EC 3.1.27.5, pancreatic ribonuclease EC 3.1.4.23: Now EC 3.1.27.1, ribonuclease T2 EC 3.1.4.24: deleted EC 3.1.4.25: Now EC 3.1.11.1, exodeoxyribonuclease I EC 3.1.4.26: deleted EC 3.1.4.27: Now EC 3.1.11.2, exodeoxyribonuclease III EC 3.1.4.28: Now EC 3.1.11.3, exodeoxyribonuclease (lambda-induced) EC 3.1.4.29: deleted EC 3.1.4.30: Now EC 3.1.21.2, deoxyribonuclease IV (phage-T4-induced) EC 3.1.4.31: Now EC 3.1.11.4 EC 3.1.4.32: deleted EC 3.1.4.33: deleted EC 3.1.4.34: deleted EC 3.1.4.35: 3′,5′-cyclic-GMP phosphodiesterase EC 3.1.4.36: Now with EC 3.1.4.43 EC 3.1.4.37: 2′,3′-cyclic-nucleotide 3'-phosphodiesterase EC 3.1.4.38: glycerophosphocholine cholinephosphodiesterase EC 3.1.4.39: alkylglycerophosphoethanolamine phosphodiesterase EC 3.1.4.40: CMP-N-acylneuraminate phosphodiesterase EC 3.1.4.41: sphingomyelin phosphodiesterase D EC 3.1.4.42: glycerol-1,2-cyclic-phosphate 2-phosphodiesterase EC 3.1.4.43: glycerophosphoinositol inositolphosphodiesterase EC 3.1.4.44: glycerophosphoinositol glycerophosphodiesterase EC 3.1.4.45: N-acetylglucosamine-1-phosphodiester α-N-acetylglucosaminidase EC 3.1.4.46: glycerophosphodiester phosphodiesterase EC 3.1.4.47: Now EC 4.6.1.14, glycosylphosphatidylinositol diacylglycerol-lyase EC 3.1.4.48: dolichylphosphate-glucose phosphodiesterase EC 3.1.4.49: dolichylphosphate-mannose phosphodiesterase EC 3.1.4.50: glycosylphosphatidylinositol phospholipase D EC 3.1.4.51: glucose-1-phospho-D-mannosylglycoprotein phosphodiesterase EC 3.1.4.52: cyclic-guanylate-specific phosphodiesterase EC 3.1.4.53: 3′,5′-cyclic-AMP phosphodiesterase EC 3.1.4.54: N-acetylphosphatidylethanolamine-hydrolysing phospholipase D EC 3.1.4.55: phosphoribosyl 1,2-cyclic phosphate phosphodiesterase EC 3.1.4.56: 7,8-dihydroneopterin 2′,3′-cyclic phosphate phosphodiesterase EC 3.1.4.57: phosphoribosyl 1,2-cyclic phosphate 1,2-diphosphodiesterase EC 3.1.4.58: RNA 2′,3′-cyclic 3′-phosphodiesterase EC 3.1.4.59: cyclic-di-AMP phosphodiesterase EC 3.1.4.60: pApA phosphodiesterase EC 3.1.4.61: cyclic 2,3-diphosphoglycerate hydrolase
EC 3.1.5: Triphosphoric Monoester Hydrolases EC 3.1.5.1: dGTPase
EC 3.1.6: Sulfuric Ester Hydrolases EC 3.1.6.1: arylsulfatase (type I) EC 3.1.6.2: steryl-sulfatase EC 3.1.6.3: glycosulfatase EC 3.1.6.4: N-acetylgalactosamine-6-sulfatase EC 3.1.6.5: deleted EC 3.1.6.6: choline-sulfatase EC 3.1.6.7: cellulose-polysulfatase EC 3.1.6.8: cerebroside-sulfatase EC 3.1.6.9: chondro-4-sulfatase EC 3.1.6.10: chondro-6-sulfatase EC 3.1.6.11: disulfoglucosamine-6-sulfatase EC 3.1.6.12: N-acetylgalactosamine-6-sulfatase EC 3.1.6.13: iduronate-2-sulfatase EC 3.1.6.14: N-acetylglucosamine-6-sulfatase EC 3.1.6.15: N-sulfoglucosamine-3-sulfatase EC 3.1.6.16: monomethyl-sulfatase EC 3.1.6.17: D-lactate-2-sulfatase EC 3.1.6.18: Glucuronate-2-sulfatase EC 3.1.6.19: (R)-specific secondary-alkylsulfatase (type III) EC 3.1.6.20: S-sulfosulfanyl-L-cysteine sulfohydrolase EC 3.1.6.21: linear primary-alkylsulfatase EC 3.1.6.22: branched primary-alkylsulfatase
EC 3.1.7: Diphosphoric Monoester Hydrolases EC 3.1.7.1: prenyl-diphosphatase EC 3.1.7.2: guanosine-3′,5′-bis(diphosphate) 3′-diphosphatase EC 3.1.7.3: monoterpenyl-diphosphatase EC 3.1.7.4: Now recognized as two enzymes EC 4.2.1.133, copal-8-ol diphosphate synthase and EC 4.2.3.141, sclareol synthase EC 3.1.7.5: geranylgeranyl diphosphate diphosphatase EC 3.1.7.6: farnesyl diphosphatase EC 3.1.7.7: Now EC 4.2.3.194, (–)-drimenol synthase EC 3.1.7.8: Now known to be a partial activity of EC 2.5.1.153, adenosine tuberculosinyltransferase. EC 3.1.7.9: Now known to be a partial activity of EC 2.5.1.153, adenosine tuberculosinyltransferase EC 3.1.7.10: (13E)-labda-7,13-dien-15-ol synthase EC 3.1.7.11: geranyl diphosphate diphosphatase EC 3.1.7.12: (+)-kolavelool synthase
EC 3.1.8: Phosphoric Triester Hydrolases EC 3.1.8.1: aryldialkylphosphatase EC 3.1.8.2: diisopropyl-fluorophosphatase
EC 3.1.11: Exodeoxyribonucleases Producing 5'-Phosphomonoesters EC 3.1.11.1: exodeoxyribonuclease I EC 3.1.11.2: exodeoxyribonuclease III EC 3.1.11.3: exodeoxyribonuclease (lambda-induced) EC 3.1.11.4: exodeoxyribonuclease (phage SP3-induced) EC 3.1.11.5: exodeoxyribonuclease V EC 3.1.11.6: exodeoxyribonuclease VII EC 3.1.11.7: Now EC 3.6.1.71, adenosine-5′-diphospho-5′-[DNA] diphosphatase EC 3.1.11.8: Now EC 3.6.1.70, guanosine-5′-diphospho-5′-[DNA] diphosphatase
EC 3.1.13: Exoribonucleases Producing 5'-Phosphomonoesters EC 3.1.13.1: exoribonuclease II EC 3.1.13.2: exoribonuclease H EC 3.1.13.3: oligonucleotidase EC 3.1.13.4: poly(A)-specific ribonuclease EC 3.1.13.5: ribonuclease D
EC 3.1.14: Exoribonucleases Producing 3'-Phosphomonoesters EC 3.1.14.1: yeast ribonuclease
EC 3.1.15: Exonucleases Active with either Ribo- or Deoxyribonucleic Acids and Producing 5'-Phosphomonoesters EC 3.1.15.1: venom exonuclease
EC 3.1.16: Exonucleases Active with either Ribo- or Deoxyribonucleic Acids and Producing 3'-Phosphomonoesters EC 3.1.16.1: spleen exonuclease
EC 3.1.21: Endodeoxyribonucleases Producing 5'-Phosphomonoesters EC 3.1.21.1: deoxyribonuclease I EC 3.1.21.2: deoxyribonuclease IV EC 3.1.21.3: type I site-specific deoxyribonuclease EC 3.1.21.4: type II site-specific deoxyribonuclease EC 3.1.21.5: type III site-specific deoxyribonuclease EC 3.1.21.6: CC-preferring endodeoxyribonuclease EC 3.1.21.7: deoxyribonuclease V EC 3.1.21.8: T4 deoxyribonuclease II EC 3.1.21.9: T4 deoxyribonuclease IV EC 3.1.21.10: crossover junction endodeoxyribonuclease
EC 3.1.22: Endodeoxyribonucleases Producing 3'-Phosphomonoesters EC 3.1.22.1: deoxyribonuclease II EC 3.1.22.2: Aspergillus deoxyribonuclease K1 EC 3.1.22.3: now EC 3.1.21.7 EC 3.1.22.4: crossover junction endodeoxyribonuclease EC 3.1.22.5: deoxyribonuclease X
EC 3.1.23: and EC 3.1.24 now EC 3.1.21.3, EC 3.1.21.4 and EC 3.1.21.5 Deleted sub-subclasses.
EC 3.1.25: Site-Specific Endodeoxyribonucleases Specific for Altered Bases EC 3.1.25.1: deoxyribonuclease (pyrimidine dimer) EC 3.1.25.2: Now EC 4.2.99.18, DNA-(apurinic or apyrimidinic site) lyase
EC 3.1.26: Endoribonucleases Producing 5'-Phosphomonoesters EC 3.1.26.1: Physarum polycephalum ribonuclease EC 3.1.26.2: ribonuclease α EC 3.1.26.3: ribonuclease III EC 3.1.26.4: ribonuclease H EC 3.1.26.5: ribonuclease P EC 3.1.26.6: ribonuclease IV EC 3.1.26.7: ribonuclease P4 EC 3.1.26.8: ribonuclease M5 EC 3.1.26.9: ribonuclease (poly-(U)-specific) EC 3.1.26.10: ribonuclease IX EC 3.1.26.11: tRNase Z EC 3.1.26.12: ribonuclease E EC 3.1.26.13: retroviral ribonuclease H
EC 3.1.27: Endoribonucleases Producing 3'-Phosphomonoesters EC 3.1.27.1: Now EC 4.6.1.19, ribonuclease T2, since the primary reaction is that of a lyase EC 3.1.27.2: Now EC 4.6.1.22, Bacillus subtilis ribonuclease, since the reaction catalysed is that of a lyase EC 3.1.27.3: Now EC 4.6.1.24, ribonuclease T1, since the primary reaction is that of a lyase EC 3.1.27.4: Now EC 4.6.1.20, ribonuclease U2, since the primary reaction is that of a lyase EC 3.1.27.5: Now EC 4.6.1.18, pancreatic ribonuclease. EC 3.1.27.6: Now EC 4.6.1.21, Enterobacter ribonuclease, since the primary reaction is that of a lyase EC 3.1.27.7: ribonuclease F EC 3.1.27.8: ribonuclease V EC 3.1.27.9: Now EC 4.6.1.16, tRNA-intron lyase EC 3.1.27.10: Now EC 4.6.1.23, ribotoxin,
EC 3.1.30: Endoribonucleases Active with either Ribo- or Deoxyribonucleic Acids and Producing 5'-Phosphomonoesters EC 3.1.30.1: Aspergillus nuclease S1 EC 3.1.30.2: Serratia marcescens nuclease
EC 3.1.31: Endoribonucleases Active with either Ribo- or Deoxyribonucleic Acids and Producing 3'-Phosphomonoesters EC 3.1.31.1: micrococcal nuclease
EC 3.2: Glycosylases
EC 3.2.1: Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds EC 3.2.1.1: α-amylase EC 3.2.1.2: β-amylase EC 3.2.1.3: glucan 1,4-α-glucosidase EC 3.2.1.4: cellulase EC 3.2.1.5: deleted EC 3.2.1.6: endo-1,3(4)-β-glucanase EC 3.2.1.7: inulinase EC 3.2.1.8: endo-1,4-β-xylanase EC 3.2.1.9: deleted EC 3.2.1.10: oligo-1,6-glucosidase EC 3.2.1.11: dextranase EC 3.2.1.12: Now included with EC 3.2.1.54, cyclomaltodextrinase EC 3.2.1.13: Now included with EC 3.2.1.54, cyclomaltodextrinase EC 3.2.1.14: chitinase EC 3.2.1.15: polygalacturonase EC 3.2.1.16: deleted EC 3.2.1.17: lysozyme EC 3.2.1.18: exo-α-sialidase EC 3.2.1.19: deleted EC 3.2.1.20: α-glucosidase EC 3.2.1.21: β-glucosidase EC 3.2.1.22: α-galactosidase EC 3.2.1.23: β-galactosidase EC 3.2.1.24: α-mannosidase EC 3.2.1.25: β-mannosidase EC 3.2.1.26: β-fructofuranosidase (invertase) EC 3.2.1.27: deleted EC 3.2.1.28: α,α-trehalase EC 3.2.1.29: Now included with EC 3.2.1.52, β-N-acetylhexosaminidase EC 3.2.1.30: Now included with EC 3.2.1.52, β-N-acetylhexosaminidase EC 3.2.1.31: β-glucuronidase EC 3.2.1.32: endo-1,3-β-xylanase EC 3.2.1.33: amylo-α-1,6-glucosidase EC 3.2.1.34: Now included with EC 3.2.1.35, hyaluronoglucosaminidase EC 3.2.1.35: hyaluronoglucosaminidase EC 3.2.1.36: hyaluronoglucuronidase EC 3.2.1.37: xylan 1,4-β-xylosidase EC 3.2.1.38: β-D-fucosidase EC 3.2.1.39: glucan endo-1,3-β-D-glucosidase EC 3.2.1.40: α-L-rhamnosidase EC 3.2.1.41: pullulanase EC 3.2.1.42: GDP-glucosidase EC 3.2.1.43: β-L-rhamnosidase EC 3.2.1.44: Now EC 3.2.1.211, endo-(13)-fucoidanase and EC 3.2.1.212, endo-(14)-fucoidanase EC 3.2.1.45: glucosylceramidase EC 3.2.1.46: galactosylceramidase EC 3.2.1.47: Now known to be catalyzed by EC 3.2.1.22, α-galactosidase EC 3.2.1.48: sucrose α-glucosidase EC 3.2.1.49: α-N-acetylgalactosaminidase EC 3.2.1.50: α-N-acetylglucosaminidase EC 3.2.1.51: α-L-fucosidase EC 3.2.1.52: β-N-acetylhexosaminidase EC 3.2.1.53: β-N-acetylgalactosaminidase EC 3.2.1.54: cyclomaltodextrinase EC 3.2.1.55: non-reducing end α-L-arabinofuranosidase EC 3.2.1.56: glucuronosyl-disulfoglucosamine glucuronidase EC 3.2.1.57: isopullulanase EC 3.2.1.58: glucan 1,3-β-glucosidase EC 3.2.1.59: glucan endo-1,3-α-glucosidase EC 3.2.1.60: glucan 1,4-α-maltotetraohydrolase EC 3.2.1.61: mycodextranase EC 3.2.1.62: glycosylceramidase EC 3.2.1.63: 1,2-α-L-fucosidase EC 3.2.1.64: 2,6-β-fructan 6-levanbiohydrolase EC 3.2.1.65: levanase EC 3.2.1.66: Deleted entry: The activity is covered by EC 3.2.1.40, α-L-rhamnosidase EC 3.2.1.67: galacturan 1,4-α-galacturonidase EC 3.2.1.68: isoamylase EC 3.2.1.69: Now included with EC 3.2.1.41, pullulanase EC 3.2.1.70: glucan 1,6-α-glucosidase EC 3.2.1.71: glucan endo-1,2-β-glucosidase EC 3.2.1.72: xylan 1,3-β-xylosidase EC 3.2.1.73: licheninase EC 3.2.1.74: glucan 1,4-β-glucosidase EC 3.2.1.75: glucan endo-1,6-β-glucosidase EC 3.2.1.76: L-iduronidase EC 3.2.1.77: mannan 1,2-(1,3)-α-mannosidase EC 3.2.1.78: mannan endo-1,4-β-mannosidase EC 3.2.1.79: Now included with EC 3.2.1.55, non-reducing end α-L-arabinofuranosidase EC 3.2.1.80: fructan β-fructosidase EC 3.2.1.81: β-agarase EC 3.2.1.82: exo-poly-α-digalacturonosidas EC 3.2.1.83: κ-carrageenase EC 3.2.1.84: glucan 1,3-α-glucosidase EC 3.2.1.85: 6-phospho-β-galactosidase EC 3.2.1.86: 6-phospho-β-glucosidase EC 3.2.1.87: capsular-polysaccharide endo-1,3-α-galactosidase EC 3.2.1.88: non-reducing end β-L-arabinopyranosidase EC 3.2.1.89: arabinogalactan endo-β-1,4-galactanase EC 3.2.1.90: Deleted, not sufficiently characterised. EC 3.2.1.91: cellulose 1,4-β-cellobiosidase (non-reducing end) EC 3.2.1.92: peptidoglycan β-N-acetylmuramidase EC 3.2.1.93: α,α-phosphotrehalase EC 3.2.1.94: glucan 1,6-α-isomaltosidase EC 3.2.1.95: dextran 1,6-α-isomaltotriosidase EC 3.2.1.96: mannosyl-glycoprotein endo-β-N-acetylglucosaminidase EC 3.2.1.97: endo-α-N-acetylgalactosaminidase EC 3.2.1.98: glucan 1,4-α-maltohexaosidase EC 3.2.1.99: arabinan endo-1,5-α-L-arabinanase EC 3.2.1.100: mannan 1,4-mannobiosidase EC 3.2.1.101: mannan endo-1,6-α-mannosidase EC 3.2.1.102: blood-group-substance endo-1,4-β-galactosidase EC 3.2.1.103: keratan-sulfate endo-1,4-β-galactosidase EC 3.2.1.104: steryl-β-glucosidase EC 3.2.1.105: 3α(S)-strictosidine β-glucosidase EC 3.2.1.106: mannosyl-oligosaccharide glucosidase EC 3.2.1.107: protein-glucosylgalactosylhydroxylysine glucosidase EC 3.2.1.108: lactase EC 3.2.1.109: endogalactosaminidase EC 3.2.1.110: identical to EC 3.2.1.97, endo-α-N-acetylgalactosaminidase EC 3.2.1.111: 1,3-α-L-fucosidase EC 3.2.1.112: 2-deoxyglucosidase EC 3.2.1.113: mannosyl-oligosaccharide 1,2-α-mannosidase EC 3.2.1.114: mannosyl-oligosaccharide 1,3-1,6-α-mannosidas EC 3.2.1.115: branched-dextran exo-1,2-α-glucosidase EC 3.2.1.116: glucan 1,4-α-maltotriohydrolase EC 3.2.1.117: amygdalin β-glucosidase EC 3.2.1.118: prunasin β-glucosidase EC 3.2.1.119: vicianin β-glucosidase EC 3.2.1.120: oligoxyloglucan β-glycosidase EC 3.2.1.121: polymannuronate hydrolase EC 3.2.1.122: maltose-6′-phosphate glucosidase EC 3.2.1.123: endoglycosylceramidase EC 3.2.1.124: 3-deoxy-2-octulosonidase EC 3.2.1.125: raucaffricine β-glucosidase EC 3.2.1.126: coniferin β-glucosidase EC 3.2.1.127: 1,6-α-L-fucosidase EC 3.2.1.128: glycyrrhizin hydrolase EC 3.2.1.129: endo-α-sialidase EC 3.2.1.130: glycoprotein endo-α-1,2-mannosidase EC 3.2.1.131: xylan α-1,2-glucuronosidase EC 3.2.1.132: chitosanase EC 3.2.1.133: glucan 1,4-α-maltohydrolase EC 3.2.1.134: difructose-anhydride synthase EC 3.2.1.135: neopullulanase EC 3.2.1.136: glucuronoarabinoxylan endo-1,4-β-xylanase EC 3.2.1.137: mannan exo-1,2-1,6-α-mannosidase EC 3.2.1.138: Now EC 4.2.2.15, anhydrosialidase EC 3.2.1.139: α-glucuronidase EC 3.2.1.140: lacto-N-biosidase EC 3.2.1.141: 4-α-D-{(1→4)-α-D-glucano}trehalose trehalohydrolase EC 3.2.1.142: limit dextrinase EC 3.2.1.143: poly(ADP-ribose) glycohydrolase EC 3.2.1.144: 3-deoxyoctulosonase EC 3.2.1.145: galactan 1,3-β-galactosidase EC 3.2.1.146: β-galactofuranosidase EC 3.2.1.147: thioglucosidase EC 3.2.1.148: The activity is most probably attributable to EC 4.4.1.21, S-ribosylhomocysteine lyase EC 3.2.1.149: β-primeverosidase EC 3.2.1.150: oligoxyloglucan reducing-end-specific cellobiohydrolase EC 3.2.1.151: xyloglucan-specific endo-β-1,4-glucanase EC 3.2.1.152: mannosylglycoprotein endo-β-mannosidase EC 3.2.1.153: fructan β-(2,1)-fructosidase EC 3.2.1.154: fructan β-(2,6)-fructosidase EC 3.2.1.155: xyloglucan-specific endo-processive β-1,4-glucanase EC 3.2.1.156: oligosaccharide reducing-end xylanase EC 3.2.1.157: ι-carrageenase EC 3.2.1.158: α-agarase EC 3.2.1.159: α-neoagaro-oligosaccharide hydrolase EC 3.2.1.160: identical to EC 3.2.1.155, xyloglucan-specific exo-β-1,4-glucanase EC 3.2.1.161: β-apiosyl-β-glucosidase EC 3.2.1.162: λ-carrageenase EC 3.2.1.163: 1,6-α-D-mannosidase EC 3.2.1.164: galactan endo-1,6-β-galactosidase EC 3.2.1.165: exo-1,4-β-D-glucosaminidase EC 3.2.1.166: heparanase EC 3.2.1.167: baicalin-β-D-glucuronidase EC 3.2.1.168: hesperidin 6-O-α-L-rhamnosyl-β-D-glucosidase EC 3.2.1.169: protein O-GlcNAcas EC 3.2.1.170: mannosylglycerate hydrolase EC 3.2.1.171: rhamnogalacturonan hydrolase EC 3.2.1.172: unsaturated rhamnogalacturonyl hydrolase EC 3.2.1.173: rhamnogalacturonan galacturonohydrolase EC 3.2.1.174: rhamnogalacturonan rhamnohydrolase EC 3.2.1.175: β-D-glucopyranosyl abscisate β-glucosidase EC 3.2.1.176: cellulose 1,4-β-cellobiosidase (reducing end) EC 3.2.1.177: α-D-xyloside xylohydrolase EC 3.2.1.178: β-porphyranase EC 3.2.1.179: gellan tetrasaccharide unsaturated glucuronyl hydrolase EC 3.2.1.180: unsaturated chondroitin disaccharide hydrolase EC 3.2.1.181: galactan endo-β-1,3-galactanase EC 3.2.1.182: 4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl glucoside β-D-glucosidase EC 3.2.1.183: UDP-N-acetylglucosamine 2-epimerase (hydrolysing) EC 3.2.1.184: UDP-N,N′-diacetylbacillosamine 2-epimerase (hydrolysing) EC 3.2.1.185: non-reducing end β-L-arabinofuranosidase EC 3.2.1.186: protodioscin 26-O-β-D-glucosidase EC 3.2.1.187: (Ara-f)3-Hyp β-L-arabinobiosidase EC 3.2.1.188: avenacosidase * EC 3.2.1.189: dioscin glycosidase (diosgenin-forming) EC 3.2.1.190: dioscin glycosidase (3-O-β-D-Glc-diosgenin-forming) EC 3.2.1.191: ginsenosidase type III EC 3.2.1.192: ginsenoside Rb1 β-glucosidase EC 3.2.1.193: ginsenosidase type I EC 3.2.1.194: ginsenosidase type IV EC 3.2.1.195: 20-O-multi-glycoside ginsenosidase EC 3.2.1.196: limit dextrin α-1,6-maltotetraose-hydrolase EC 3.2.1.197: β-1,2-mannosidase EC 3.2.1.198: α-mannan endo-1,2-α-mannanase EC 3.2.1.199: sulfoquinovosidase EC 3.2.1.200: exo-chitinase (non-reducing end) EC 3.2.1.201: exo-chitinase (reducing end) EC 3.2.1.202: endo-chitodextinase EC 3.2.1.203: carboxymethylcellulase EC 3.2.1.204: 1,3-α-isomaltosidase EC 3.2.1.205: isomaltose glucohydrolase EC 3.2.1.206: oleuropein β-glucosidase EC 3.2.1.207: mannosyl-oligosaccharide α-1,3-glucosidase EC 3.2.1.208: glucosylglycerate hydrolase EC 3.2.1.209: endoplasmic reticulum Man9GlcNAc2 1,2-α-mannosidase EC 3.2.1.210: endoplasmic reticulum Man8GlcNAc2 1,2-α-mannosidase EC 3.2.1.211: endo-(1→3)-fucoidanase EC 3.2.1.212: endo-(1→4)-fucoidanase EC 3.2.1.213: galactan exo-1,6-β-galactobiohydrolase (non-reducing end) EC 3.2.1.214: exo β-1,2-glucooligosaccharide sophorohydrolase (non-reducing end)
EC 3.2.2: Hydrolysing N-Glycosyl Compounds EC 3.2.2.1: purine nucleosidase EC 3.2.2.2: inosine nucleosidase EC 3.2.2.3: uridine nucleosidase EC 3.2.2.4: AMP nucleosidase EC 3.2.2.5: NAD+ glycohydrolase EC 3.2.2.6: ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase EC 3.2.2.7: adenosine nucleosidase EC 3.2.2.8: ribosylpyrimidine nucleosidase EC 3.2.2.9: adenosylhomocysteine nucleosidase EC 3.2.2.10: pyrimidine-5′-nucleotide nucleosidase EC 3.2.2.11: β-aspartyl-N-acetylglucosaminidase EC 3.2.2.12: inosinate nucleosidase EC 3.2.2.13: 1-methyladenosine nucleosidase EC 3.2.2.14: NMN nucleosidase EC 3.2.2.15: DNA-deoxyinosine glycosylase EC 3.2.2.16: methylthioadenosine nucleosidase EC 3.2.2.17: deoxyribodipyrimidine endonucleosidase EC 3.2.2.18: Now included with EC 3.5.1.52, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase EC 3.2.2.19: ADP-ribosylarginine hydrolase EC 3.2.2.20: DNA-3-methyladenine glycosylase I EC 3.2.2.21: DNA-3-methyladenine glycosylase II, MAG1 EC 3.2.2.22: rRNA 'N-glycosylase EC 3.2.2.23: DNA-formamidopyrimidine glycosylase EC 3.2.2.24: ADP-ribosyl-[dinitrogen reductase] hydrolase EC 3.2.2.25: N-methyl nucleosidase EC 3.2.2.26: futalosine hydrolase EC 3.2.2.27: uracil-DNA glycosylase EC 3.2.2.28: double-stranded uracil-DNA glycosylase EC 3.2.2.29: thymine-DNA glycosylase EC 3.2.2.30: aminodeoxyfutalosine nucleosidase EC 3.2.2.31: adenine glycosylase
EC 3.2.3: Hydrolysing S-Glycosyl Compounds Deleted sub-subclass
EC 3.3: Acting on Ether Bonds
EC 3.3.1: Thioether and trialkylsulfonium hydrolases EC 3.3.1.1: adenosylhomocysteinase EC 3.3.1.2: S-adenosyl-L-methionine hydrolase (L-homoserine-forming) EC 3.3.1.3: The activity is most probably attributable to EC 4.4.1.21, S-ribosylhomocysteine lyase
EC 3.3.2: Ether Hydrolases EC 3.3.2.1: isochorismatase EC 3.3.2.2: alkenylglycerophosphocholine hydrolase EC 3.3.2.3: Now known to comprise two enzymes, microsomal epoxide hydrolase (EC 3.3.2.9) and soluble epoxide hydrolase (EC 3.3.2.10) EC 3.3.2.4: trans-epoxysuccinate hydrolase EC 3.3.2.5: Now included in EC 3.3.2.2, lysoplasmalogenase EC 3.3.2.6: leukotriene-A4 hydrolase EC 3.3.2.7: hepoxilin-epoxide hydrolase EC 3.3.2.8: limonene-1,2-epoxide hydrolase EC 3.3.2.9: microsomal epoxide hydrolase EC 3.3.2.10: soluble epoxide hydrolase EC 3.3.2.11: cholesterol-5,6-oxide hydrolase EC 3.3.2.12: oxepin-CoA hydrolase EC 3.3.2.13: chorismatase EC 3.3.2.14: 2,4-dinitroanisole O-demethylase EC 3.3.2.15: trans-2,3-dihydro-3-hydroxyanthranilic acid synthase
EC 3.4: Acting on peptide bonds – Peptidase
EC 3.4.1 α-amino acyl peptide hydrolases (discontinued) EC 3.4.1.1: Now EC 3.4.11.1, leucyl aminopeptidase EC 3.4.1.2: Now EC 3.4.11.2, membrane alanyl aminopeptidase EC 3.4.1.3: Now EC 3.4.11.4, tripeptide aminopeptidase EC 3.4.1.4: Now EC 3.4.11.5, prolyl aminopeptidase
EC 3.4.2 Peptidyl amino acid hydrolases (discontinued) EC 3.4.2.1: Now EC 3.4.17.1, carboxypeptidase A EC 3.4.2.2: Now EC 3.4.17.2, carboxypeptidase B EC 3.4.2.3: Now EC 3.4.17.4, Gly-Xaa carboxypeptidase
EC 3.4.3: Dipeptide hydrolases (deleted sub-subclass) EC 3.4.3.1: Now EC 3.4.13.18, cytosol nonspecific dipeptidase EC 3.4.3.2: Now EC 3.4.13.18, cytosol nonspecific dipeptidase EC 3.4.3.3: Now EC 3.4.13.3, Xaa-His dipeptidase EC 3.4.3.4: Now EC 3.4.13.5, Xaa-methyl-His dipeptidase EC 3.4.3.5: Now EC 3.4.11.2, membrane alanyl aminopeptidase EC 3.4.3.6: Now EC 3.4.13.18, cytosol nonspecific dipeptidase EC 3.4.3.7: Now EC 3.4.13.9, Xaa-Pro dipeptidase
EC 3.4.4 Peptidyl Peptide Hydrolases (discontinued) EC 3.4.4.1: Now EC 3.4.23.1, pepsin A EC 3.4.4.2: Now EC 3.4.23.2, pepsin B EC 3.4.4.3: Now EC 3.4.23.4, chymosin EC 3.4.4.4: Now EC 3.4.21.4, trypsin EC 3.4.4.5: Now EC 3.4.21.1, chymotrypsin EC 3.4.4.6: Now EC 3.4.21.1, chymotrypsin EC 3.4.4.7: Now covered by EC 3.4.21.36, pancreatic elastase and EC 3.4.21.37, leukocyte elastase EC 3.4.4.8: Now EC 3.4.21.9, enteropeptidase EC 3.4.4.9: Now EC 3.4.14.1, dipeptidyl-peptidase I EC 3.4.4.10: Now EC 3.4.22.2, papain EC 3.4.4.11: Now EC 3.4.22.6, chymopapain EC 3.4.4.12: Now EC 3.4.22.3, ficain EC 3.4.4.13: Now EC 3.4.21.5, thrombin EC 3.4.4.14: Now EC 3.4.21.7, plasmin EC 3.4.4.15: Now EC 3.4.23.15, renin EC 3.4.4.16: Now covered by the microbial serine proteinases EC 3.4.21.62 (subtilisin), EC 3.4.21.63 (oryzin), EC 3.4.21.64 (endopeptidase K), EC 3.4.21.65 (thermomycolin), EC 3.4.21.66 (thermitase) and EC 3.4.21.67 (endopeptidase So) EC 3.4.4.17: Now covered by the microbial aspartic proteinases EC 3.4.23.20 (penicillopepsin), EC 3.4.23.21 (rhizopuspepsin), EC 3.4.23.22 (endothiapepsin), EC 3.4.23.23 (mucorpepsin), EC 3.4.23.24 (candidapepsin), EC 3.4.23.25 (saccharopepsin), EC 3.4.23.26 (rhodotorulapepsin), EC 3.4.21.103 (physarolisin), EC 3.4.23.28 (acrocylindropepsin), EC 3.4.23.29 (polyporopepsin) and EC 3.4.23.30 (pycnoporopepsin) EC 3.4.4.18: Now EC 3.4.22.10, streptopain EC 3.4.4.19: Now EC 3.4.24.3, microbial collagenase EC 3.4.4.20: Now EC 3.4.22.8, clostripain EC 3.4.4.21: Now EC 3.4.21.34 (plasma kallikrein) and EC 3.4.21.35 (tissue kallikrein) EC 3.4.4.22: Now EC 3.4.23.3, gastricsin EC 3.4.4.23: Now EC 3.4.23.5, cathepsin D EC 3.4.4.24: Now covered by EC 3.4.22.32 (stem bromelain) and EC 3.4.22.33 (fruit bromelain) EC 3.4.4.25: deleted
EC 3.4.11 Aminopeptidases EC 3.4.11.1: leucyl aminopeptidase EC 3.4.11.2: membrane alanyl aminopeptidase EC 3.4.11.3: cystinyl aminopeptidase EC 3.4.11.4: tripeptide aminopeptidase EC 3.4.11.5: prolyl aminopeptidase EC 3.4.11.6: aminopeptidase B EC 3.4.11.7: glutamyl aminopeptidase EC 3.4.11.8: Now EC 3.4.19.3, pyroglutamyl-peptidase I EC 3.4.11.9: Xaa-Pro aminopeptidase EC 3.4.11.10: bacterial leucyl aminopeptidase EC 3.4.11.11: Deleted EC 3.4.11.12: Deleted EC 3.4.11.13: Clostridial aminopeptidase EC 3.4.11.14: cytosol alanyl aminopeptidase EC 3.4.11.15: aminopeptidase Y EC 3.4.11.16: Xaa-Trp aminopeptidase EC 3.4.11.17: tryptophanyl aminopeptidase EC 3.4.11.18: methionyl aminopeptidase EC 3.4.11.19: D-stereospecific aminopeptidase EC 3.4.11.20: aminopeptidase Ey EC 3.4.11.21: aspartyl aminopeptidase EC 3.4.11.22: aminopeptidase I EC 3.4.11.23: PepB aminopeptidase EC 3.4.11.24: aminopeptidase S EC 3.4.11.25: β-peptidyl aminopeptidase EC 3.4.11.26: intermediate cleaving peptidase 55
EC 3.4.12 Peptidylamino-acid hydrolases or acylamino-acid hydrolases (deleted sub-subclass) EC 3.4.12.1: Now EC 3.4.16.5 (carboxypeptidase C) and EC 3.4.16.6 (carboxypeptidase D) EC 3.4.12.2: Now EC 3.4.17.1, carboxypeptidase A EC 3.4.12.3: Now EC 3.4.17.2, carboxypeptidase B EC 3.4.12.4: Now EC 3.4.16.2, lysosomal Pro-Xaa carboxypeptidase EC 3.4.12.5: Now EC 3.5.1.28, N-acetylmuramoyl-L-alanine amidase EC 3.4.12.6: Now EC 3.4.17.8, muramoyl-pentapeptidase carboxypeptidase EC 3.4.12.7: Now EC 3.4.17.3, lysine carboxypeptidase EC 3.4.12.8: Now EC 3.4.17.4, Gly-Xaa carboxypeptidase EC 3.4.12.9: aspartate carboxypeptidase EC 3.4.12.10: Now EC 3.4.19.9, γ-glutamyl hydrolase EC 3.4.12.11: Now EC 3.4.17.6, alanine carboxypeptidase EC 3.4.12.12: Now EC 3.4.16.5 (carboxypeptidase C) and EC 3.4.16.6 (carboxypeptidase D) EC 3.4.12.13: γ-glutamylglutamate carboxypeptidase
EC 3.4.13 Dipeptidases EC 3.4.13.1: Now EC 3.4.13.18, cytosol nonspecific dipeptidase EC 3.4.13.2: Now EC 3.4.13.18, cytosol nonspecific dipeptidase EC 3.4.13.3: The activity is covered by EC .4.13.18, cytosol nonspecific dipeptidase and EC 3.4.13.20, β-Ala-His dipeptidase EC 3.4.13.4: Xaa-Arg dipeptidase EC 3.4.13.5: Xaa-methyl-His dipeptidase EC 3.4.13.6: Now EC 3.4.11.2, membrane alanyl aminopeptidase EC 3.4.13.7: Glu-Glu dipeptidase EC 3.4.13.8: Now EC 3.4.13.18, cytosol nonspecific dipeptidase EC 3.4.13.9: Xaa-Pro dipeptidase EC 3.4.13.10: Now EC 3.4.19.5, β-aspartyl-peptidase EC 3.4.13.11: Now EC 3.4.13.19, membrane dipeptidase EC 3.4.13.12: Met-Xaa dipeptidase EC 3.4.13.13: Now EC 3.4.13.3, Xaa-His dipeptidase EC 3.4.13.14: Deleted EC 3.4.13.15: Now EC 3.4.13.18, cytosol nonspecific dipeptidase EC 3.4.13.16: Deleted EC 3.4.13.17: non-stereospecific dipeptidase EC 3.4.13.18: cytosol nonspecific dipeptidase EC 3.4.13.19: membrane dipeptidase EC 3.4.13.20: β-Ala-His dipeptidase EC 3.4.13.21: dipeptidase E EC 3.4.13.22: D-Ala-D-Ala dipeptidase EC 3.4.13.23: cysteinylglycine-S-conjugate dipeptidase
EC 3.4.14 Dipeptidyl peptidases and tripeptidyl peptidases EC 3.4.14.1: dipeptidyl-peptidase I EC 3.4.14.2: dipeptidyl-peptidase II EC 3.4.14.3: Now EC 3.4.19.1, acylaminoacyl-peptidase EC 3.4.14.4: dipeptidyl-peptidase III EC 3.4.14.5: dipeptidyl-peptidase IV EC 3.4.14.6: dipeptidyl-dipeptidase EC 3.4.14.7: Deleted EC 3.4.14.8: Now EC 3.4.14.10, tripeptidyl-peptidase II EC 3.4.14.9: tripeptidyl-peptidase I EC 3.4.14.10: tripeptidyl-peptidase II EC 3.4.14.11: Xaa-Pro dipeptidyl-peptidase EC 3.4.14.12: Xaa-Xaa-Pro tripeptidyl-peptidase EC 3.4.14.13: γ-D-glutamyl-Llysine dipeptidyl-peptidase EC 3.4.14.14: [mycofactocin precursor peptide] peptidase
EC 3.4.15 Peptidyl dipeptidases EC 3.4.15.1: peptidyl-dipeptidase A EC 3.4.15.2: Now EC 3.4.19.2, peptidyl-glycinamidase EC 3.4.15.3: Now EC 3.4.15.5, peptidyl-dipeptidase Dcp EC 3.4.15.4: Peptidyl-dipeptidase B EC 3.4.15.5: Peptidyl-dipeptidase Dcp EC 3.4.15.6: cyanophycinase
EC 3.4.16 Serine type carboxypeptidases EC 3.4.16.1: Transferred entry: serine carboxypeptidase. Now EC 3.4.16.6, carboxypeptidase D EC 3.4.16.2: lysosomal Pro-Xaa carboxypeptidase EC 3.4.16.3: Now included with EC 3.4.16.5, carboxypeptidase C EC 3.4.16.4: serine-type D-Ala-D-Ala carboxypeptidase EC 3.4.16.5: carboxypeptidase C EC 3.4.16.6: carboxypeptidase D
EC 3.4.17 Metallocarboxypeptidases EC 3.4.17.1: carboxypeptidase A EC 3.4.17.2: carboxypeptidase B EC 3.4.17.3: lysine carboxypeptidase EC 3.4.17.4: Gly-Xaa carboxypeptidase EC 3.4.17.5: Deleted EC 3.4.17.6: alanine carboxypeptidase EC 3.4.17.7: Now EC 3.5.1.28, N-acetylmuramoyl-L-alanine amidase EC 3.4.17.8: muramoylpentapeptide carboxypeptidase EC 3.4.17.9: Now included with EC 3.4.17.4, Gly-Xaa carboxypeptidase EC 3.4.17.10: carboxypeptidase E EC 3.4.17.11: glutamate carboxypeptidase EC 3.4.17.12: carboxypeptidase M EC 3.4.17.13: Muramoyltetrapeptide carboxypeptidase EC 3.4.17.14: zinc D-Ala-D-Ala carboxypeptidase EC 3.4.17.15: carboxypeptidase A2 EC 3.4.17.16: membrane Pro-Xaa carboxypeptidase EC 3.4.17.17: tubulinyl-Tyr carboxypeptidase EC 3.4.17.18: carboxypeptidase T EC 3.4.17.19: Carboxypeptidase Taq EC 3.4.17.20: carboxypeptidase U EC 3.4.17.21: Glutamate carboxypeptidase I