This list contains a list of EC numbers for the fourth group, EC 4, lyases, placed in numerical order as determined by the Nomenclature Committee of the International Union of Biochemistry and Molecular Biology. All official information is tabulated at the website of the committee. The database is developed and maintained by Andrew McDonald.
EC 4.1: Carbon-Carbon Lyases
EC 4.1.1: Carboxy-lyases EC 4.1.1.1: pyruvate decarboxylase EC 4.1.1.2: oxalate decarboxylase EC 4.1.1.3: Now recognized to be two enzymes EC 7.2.4.2 [oxaloacetate decarboxylase (Na+ extruding)] and EC 4.1.1.112 (oxaloacetate decarboxylase). EC 4.1.1.4: acetoacetate decarboxylase EC 4.1.1.5: acetolactate decarboxylase EC 4.1.1.6: cis-aconitate decarboxylase EC 4.1.1.7: benzoylformate decarboxylase EC 4.1.1.8: oxalyl-CoA decarboxylase EC 4.1.1.9: malonyl-CoA decarboxylase EC 4.1.1.10: Now included with EC 4.1.1.12, aspartate 4-decarboxylase EC 4.1.1.11: aspartate 1-decarboxylase EC 4.1.1.12: aspartate 4-decarboxylase EC 4.1.1.13: deleted EC 4.1.1.14: valine decarboxylase EC 4.1.1.15: glutamate decarboxylase EC 4.1.1.16: hydroxyglutamate decarboxylase EC 4.1.1.17: ornithine decarboxylase EC 4.1.1.18: lysine decarboxylase EC 4.1.1.19: arginine decarboxylase EC 4.1.1.20: diaminopimelate decarboxylase EC 4.1.1.21: phosphoribosylaminoimidazole carboxylase EC 4.1.1.22: histidine decarboxylase EC 4.1.1.23: orotidine-5′-phosphate decarboxylase EC 4.1.1.24: aminobenzoate decarboxylase EC 4.1.1.25: tyrosine decarboxylase EC 4.1.1.26: Now included with EC 4.1.1.28 aromatic-L-amino-acid decarboxylase EC 4.1.1.27: Now included with EC 4.1.1.28 aromatic-L-amino-acid decarboxylase EC 4.1.1.28: aromatic-L-amino-acid decarboxylase EC 4.1.1.29: sulfoalanine decarboxylase EC 4.1.1.30: pantothenoylcysteine decarboxylase EC 4.1.1.31: phosphoenolpyruvate carboxylase EC 4.1.1.32: phosphoenolpyruvate carboxykinase (GTP) EC 4.1.1.33: diphosphomevalonate decarboxylase EC 4.1.1.34: dehydro-L-gulonate decarboxylase EC 4.1.1.35: UDP-glucuronate decarboxylase EC 4.1.1.36: phosphopantothenoylcysteine decarboxylase EC 4.1.1.37: uroporphyrinogen decarboxylase EC 4.1.1.38: phosphoenolpyruvate carboxykinase (diphosphate) EC 4.1.1.39: ribulose-bisphosphate carboxylase EC 4.1.1.40: hydroxypyruvate decarboxylase EC 4.1.1.41: Now EC 7.2.4.3, (S)-methylmalonyl-CoA decarboxylase EC 4.1.1.42: carnitine decarboxylase EC 4.1.1.43: phenylpyruvate decarboxylase EC 4.1.1.44: 4-carboxymuconolactone decarboxylase EC 4.1.1.45: aminocarboxymuconate-semialdehyde decarboxylase EC 4.1.1.46: o-pyrocatechuate decarboxylase EC 4.1.1.47: tartronate-semialdehyde synthase EC 4.1.1.48: indole-3-glycerol-phosphate synthase EC 4.1.1.49: phosphoenolpyruvate carboxykinase (ATP) EC 4.1.1.50: adenosylmethionine decarboxylase EC 4.1.1.51: 3-hydroxy-2-methylpyridine-4,5-dicarboxylate 4-decarboxylase EC 4.1.1.52: 6-methylsalicylate decarboxylase EC 4.1.1.53: phenylalanine decarboxylase EC 4.1.1.54: dihydroxyfumarate decarboxylase EC 4.1.1.55: 4,5-dihydroxyphthalate decarboxylase EC 4.1.1.56: 3-oxolaurate decarboxylase EC 4.1.1.57: methionine decarboxylase EC 4.1.1.58: orsellinate decarboxylase EC 4.1.1.59: gallate decarboxylase EC 4.1.1.60: stipitatonate decarboxylase EC 4.1.1.61: 4-hydroxybenzoate decarboxylase EC 4.1.1.62: gentisate decarboxylase EC 4.1.1.63: protocatechuate decarboxylase EC 4.1.1.64: 2,2-dialkylglycine decarboxylase (pyruvate) EC 4.1.1.65: phosphatidylserine decarboxylase EC 4.1.1.66: uracil-5-carboxylate decarboxylase EC 4.1.1.67: UDP-galacturonate decarboxylase EC 4.1.1.68: 5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase EC 4.1.1.69: 3,4-dihydroxyphthalate decarboxylase EC 4.1.1.70: Now EC 7.2.4.5, glutaconyl-CoA decarboxylase EC 4.1.1.71: 2-oxoglutarate decarboxylase EC 4.1.1.72: branched-chain-2-oxoacid decarboxylase EC 4.1.1.73: tartrate decarboxylase EC 4.1.1.74: indolepyruvate decarboxylase EC 4.1.1.75: 5-guanidino-2-oxopentanoate decarboxylase EC 4.1.1.76: arylmalonate decarboxylase EC 4.1.1.77: 4-oxalocrotonate decarboxylase EC 4.1.1.78: acetylenedicarboxylate decarboxylase EC 4.1.1.79: sulfopyruvate decarboxylase EC 4.1.1.80: 4-hydroxyphenylpyruvate decarboxylase EC 4.1.1.81: threonine-phosphate decarboxylase EC 4.1.1.82: phosphonopyruvate decarboxylase EC 4.1.1.83: 4-hydroxyphenylacetate decarboxylase EC 4.1.1.84: D-dopachrome decarboxylase EC 4.1.1.85: 3-dehydro-L-gulonate-6-phosphate decarboxylase EC 4.1.1.86: diaminobutyrate decarboxylase EC 4.1.1.87: malonyl-S-ACP decarboxylase EC 4.1.1.88: biotin-independent malonate decarboxylase EC 4.1.1.89: Now EC 7.2.4.4 EC 7.2.4.4, biotin-dependent malonate decarboxylase EC 4.1.1.90: peptidyl-glutamate 4-carboxylase EC 4.1.1.91: salicylate decarboxylase EC 4.1.1.92: indole-3-carboxylate decarboxylase EC 4.1.1.93: pyrrole-2-carboxylate decarboxylase EC 4.1.1.94: ethylmalonyl-CoA decarboxylase EC 4.1.1.95: L-glutamyl-[BtrI acyl-carrier protein] decarboxylase EC 4.1.1.96: carboxynorspermidine decarboxylase EC 4.1.1.97: 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase EC 4.1.1.98: 4-hydroxy-3-polyprenylbenzoate decarboxylase EC 4.1.1.99: phosphomevalonate decarboxylase EC 4.1.1.100: prephenate decarboxylase EC 4.1.1.101: malolactic enzyme EC 4.1.1.102: phenacrylate decarboxylase EC 4.1.1.103: γ-resorcylate decarboxylase EC 4.1.1.104: 3-dehydro-4-phosphotetronate decarboxylase EC 4.1.1.105: L-tryptophan decarboxylase EC 4.1.1.106: fatty acid photodecarboxylase EC 4.1.1.107: 3,4-dihydroxyphenylacetaldehyde synthase EC 4.1.1.108: 4-hydroxyphenylacetaldehyde synthase EC 4.1.1.109: phenylacetaldehyde synthase EC 4.1.1.110: bisphosphomevalonate decarboxylase EC 4.1.1.111: siroheme decarboxylase EC 4.1.1.112: oxaloacetate decarboxylase EC 4.1.1.113: trans-aconitate decarboxylase EC 4.1.1.114: cis-3-alkyl-4-alkyloxetan-2-one decarboxylase EC 4.1.1.115: indoleacetate decarboxylase EC 4.1.1.116: D-ornithine/D-lysine decarboxylase EC 4.1.1.117: [[2-[(L-alanin-3-ylcarbamoyl)methyl]-2-hydroxybutanedioate decarboxylase|2-[(L-alanin-3-ylcarbamoyl)methyl]-2-hydroxybutanedioate decarboxylase]] EC 4.1.1.118: isophthalyl-CoA decarboxylase EC 4.1.1.119: phenylacetate decarboxylase EC 4.1.1.120: 3-oxoisoapionate decarboxylase EC 4.1.1.121: 3-oxoisoapionate-4-phosphate decarboxylase
EC 4.1.2: Aldehyde-lyases EC 4.1.2.1: Now included with EC 4.1.3.16 4-hydroxy-2-oxoglutarate aldolase EC 4.1.2.2: ketotetrose-phosphate aldolase EC 4.1.2.3: deleted, was pentosealdolase. EC 4.1.2.4: deoxyribose-phosphate aldolase EC 4.1.2.5: L-threonine aldolase EC 4.1.2.6: Deleted, reaction is due to EC 2.1.2.1, glycine hydroxymethyltransferase EC 4.1.2.7: Now included with EC 4.1.2.13 fructose-bisphosphate aldolase EC 4.1.2.8: indole-3-glycerol-phosphate lyase EC 4.1.2.9: phosphoketolase EC 4.1.2.10: (R)-mandelonitrile lyase EC 4.1.2.11: hydroxymandelonitrile lyase EC 4.1.2.12: 2-dehydropantoate aldolase EC 4.1.2.13: fructose-bisphosphate aldolase EC 4.1.2.14: 2-dehydro-3-deoxy-phosphogluconate aldolase EC 4.1.2.15: Now EC 2.5.1.54, 3-deoxy-7-phosphoheptulonate synthase EC 4.1.2.16: Now EC 2.5.1.55, 3-deoxy-8-phosphooctulonate synthase EC 4.1.2.17: L-fuculose-phosphate aldolase EC 4.1.2.18: 2-dehydro-3-deoxy-L-pentonate aldolase EC 4.1.2.19: rhamnulose-1-phosphate aldolase EC 4.1.2.20: 2-dehydro-3-deoxyglucarate aldolase EC 4.1.2.21: 2-dehydro-3-deoxy-6-phosphogalactonate aldolase EC 4.1.2.22: fructose-6-phosphate phosphoketolase EC 4.1.2.23: 3-deoxy-D-manno-octulosonate aldolase EC 4.1.2.24: dimethylaniline-N-oxide aldolase EC 4.1.2.25: dihydroneopterin aldolase EC 4.1.2.26: phenylserine aldolase EC 4.1.2.27: sphinganine-1-phosphate aldolase EC 4.1.2.28: 2-dehydro-3-deoxy-D-pentonate aldolase EC 4.1.2.29: 5-dehydro-2-deoxyphosphogluconate aldolase EC 4.1.2.30: Now EC 1.14.14.32}, 17α-hydroxyprogesterone deacetylase EC 4.1.2.31: Now included with EC 4.1.3.16 4-hydroxy-2-oxoglutarate aldolase EC 4.1.2.32: trimethylamine-oxide aldolase EC 4.1.2.33: fucosterol-epoxide lyase EC 4.1.2.34: 4-(2-carboxyphenyl)-2-oxobut-3-enoate aldolase EC 4.1.2.35: propioin synthase EC 4.1.2.36: lactate aldolase EC 4.1.2.37: Now covered by EC 4.1.2.46 [aliphatic (R)-hydroxynitrile lyase] and EC 4.1.2.47 [(S)-hydroxynitrile ketone-lyase (cyanide forming)] EC 4.1.2.38: benzoin aldolase EC 4.1.2.39: Deleted, identical to EC 4.1.2.37, hydroxynitrilase EC 4.1.2.40: tagatose-bisphosphate aldolase EC 4.1.2.41: Now included with EC 4.1.2.61, feruloyl-CoA hydratase/lyase EC 4.1.2.42: D-threonine aldolase EC 4.1.2.43: 3-hexulose-6-phosphate synthase EC 4.1.2.44: benzoyl-CoA-dihydrodiol lyase EC 4.1.2.45: trans-o-hydroxybenzylidenepyruvate hydratase-aldolase EC 4.1.2.46: aliphatic (R)-hydroxynitrile lyase EC 4.1.2.47: (S)-hydroxynitrile lyase EC 4.1.2.48: low-specificity L-threonine aldolase EC 4.1.2.49: L-allo-threonine aldolase EC 4.1.2.50: 6-carboxytetrahydropterin synthase * EC 4.1.2.50: 6-carboxytetrahydropterin synthase * EC 4.1.2.51: 2-dehydro-3-deoxy-D-gluconate aldolase * EC 4.1.2.52: 4-hydroxy-2-oxoheptanedioate aldolase * EC 4.1.2.53: 2-keto-3-deoxy-L-rhamnonate aldolase * EC 4.1.2.54: L-threo-3-deoxy-hexylosonate aldolase * EC 4.1.2.55: 2-dehydro-3-deoxy-phosphogluconate/2-dehydro-3-deoxy-6-phosphogalactonate aldolase * EC 4.1.2.56: 2-amino-4,5-dihydroxy-6-oxo-7-(phosphooxy)heptanoate synthase * EC 4.1.2.57: sulfofructosephosphate aldolase * EC 4.1.2.58: 2-dehydro-3,6-dideoxy-6-sulfogluconate aldolase * EC 4.1.2.59: dihydroneopterin phosphate aldolase * EC 4.1.2.60: dihydroneopterin triphosphate aldolase * EC 4.1.2.61: feruloyl-CoA hydratase/lyase * EC 4.1.2.62: 5-deoxyribulose 1-phosphate aldolase * EC 4.1.2.63: 2-hydroxyacyl-CoA lyase
EC 4.1.3: Oxo-Acid-Lyases EC 4.1.3.1: isocitrate lyase EC 4.1.3.2: Now EC 2.3.3.9, malate synthase EC 4.1.3.3: N-acetylneuraminate lyase EC 4.1.3.4: hydroxymethylglutaryl-CoA lyase EC 4.1.3.5: Now EC 2.3.3.10, hydroxymethylglutaryl-CoA synthase EC 4.1.3.6: citrate (pro-3S)-lyase EC 4.1.3.7: Now EC 2.3.3.1, citrate (Si)-synthase EC 4.1.3.8: Now EC 2.3.3.8, ATP citrate synthase EC 4.1.3.9: Now EC 2.3.3.11, 2-hydroxyglutarate synthase EC 4.1.3.10: Now EC 2.3.3.7, 3-ethylmalate synthase EC 4.1.3.11: Now EC 2.3.3.12, 3-propylmalate synthase EC 4.1.3.12: Now EC 2.3.3.13, 2-isopropylmalate synthase EC 4.1.3.13: oxalomalate lyase EC 4.1.3.14: L-erythro-3-hydroxyaspartate aldolase EC 4.1.3.15: Now EC 2.2.1.5, 2-hydroxy-3-oxoadipate synthase EC 4.1.3.16: 4-hydroxy-2-oxoglutarate aldolase EC 4.1.3.17: 4-hydroxy-4-methyl-2-oxoglutarate aldolase EC 4.1.3.18: Now EC 2.2.1.6, acetolactate synthase EC 4.1.3.19: Now EC 2.5.1.56 EC 4.1.3.20: Now EC 2.5.1.57 EC 4.1.3.21: Now EC 2.3.3.14 EC 4.1.3.22: citramalate lyase EC 4.1.3.23: Now EC 2.3.3.2, decylcitrate synthase EC 4.1.3.24: malyl-CoA lyase EC 4.1.3.25: (S)-citramalyl-CoA lyase EC 4.1.3.26: 3-hydroxy-3-isohexenylglutaryl-CoA lyase EC 4.1.3.27: anthranilate synthase EC 4.1.3.28: Now EC 2.3.3.3, citrate (Re)-synthase EC 4.1.3.29: Now EC 2.3.3.4, decylhomocitrate synthase EC 4.1.3.30: methylisocitrate lyase EC 4.1.3.31: Now EC 2.3.3.5, 2-methylcitrate synthase EC 4.1.3.32: 2,3-dimethylmalate lyase EC 4.1.3.33: Now EC 2.3.3.6 EC 4.1.3.34: citryl-CoA lyase EC 4.1.3.35: (1-hydroxycyclohexan-1-yl)acetyl-CoA lyase EC 4.1.3.36: naphthoate synthase EC 4.1.3.37: Now EC 2.2.1.7, 1-deoxy-D-xylulose 5-phosphate synthase EC 4.1.3.38: aminodeoxychorismate lyase EC 4.1.3.39: 4-hydroxy-2-oxovalerate aldolase EC 4.1.3.40: chorismate lyase EC 4.1.3.41: 3-hydroxy-D-aspartate aldolase EC 4.1.3.42: (4S)-4-hydroxy-2-oxoglutarate aldolase EC 4.1.3.43: 4-hydroxy-2-oxohexanoate aldolase EC 4.1.3.44: tRNA 4-demethylwyosine synthase (AdoMet-dependent) EC 4.1.3.45: 3-hydroxybenzoate synthase EC 4.1.3.46: (R)-citramalyl-CoA lyase
EC 4.1.99: Other Carbon-Carbon Lyases EC 4.1.99.1: tryptophanase EC 4.1.99.2: tyrosine phenol-lyase EC 4.1.99.3: deoxyribodipyrimidine photo-lyase EC 4.1.99.4: Now EC 3.5.99.7, 1-aminocyclopropane-1-carboxylate deaminase EC 4.1.99.5: octadecanal decarbonylase EC 4.1.99.6: Now EC 4.2.3.6, trichodiene synthase EC 4.1.99.7: Now EC 4.2.3.9, aristolochene synthase EC 4.1.99.8: Now EC 4.2.3.14, pinene synthase EC 4.1.99.9: Now EC 4.2.3.15, myrcene synthase EC 4.1.99.10: Now EC 4.2.3.16, (4S)-limonene synthase EC 4.1.99.11: benzylsuccinate synthase EC 4.1.99.12: 3,4-dihydroxy-2-butanone-4-phosphate synthase EC 4.1.99.13: (6-4)DNA photolyase EC 4.1.99.14: spore photoproduct lyase EC 4.1.99.15: The activity is covered by EC 4.1.99.14, spore photoproduct lyase EC 4.1.99.16: geosmin synthase EC 4.1.99.17: phosphomethylpyrimidine synthase EC 4.1.99.18: Now known to be catalysed by the combined effect of EC 4.1.99.22, GTP 3,8-cyclase, and EC 4.6.1.17, cyclic pyranopterin monophosphate synthase EC 4.1.99.19: 2-iminoacetate synthase EC 4.1.99.20: 3-amino-4-hydroxybenzoate synthase EC 4.1.99.21: Now EC 4.2.3.153 (5-formylfuran-3-yl)methyl phosphate synthase. EC 4.1.99.22: GTP 3′,8-cyclase EC 4.1.99.23: 5-hydroxybenzimidazole synthase EC 4.1.99.24: L-tyrosine isonitrile synthase EC 4.1.99.25: L-tryptophan isonitrile synthase EC 4.1.99.26: [[3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one|3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one]]
EC 4.2: Carbon-Oxygen Lyases
EC 4.2.1: Hydro-lyases EC 4.2.1.1: carbonic anhydrase EC 4.2.1.2: fumarate hydratase EC 4.2.1.3: aconitate hydratase EC 4.2.1.4: Now known to be a partial reaction catalysed by EC 4.2.1.3, aconitate hydratase EC 4.2.1.5: arabinonate dehydratase EC 4.2.1.6: galactonate dehydratase EC 4.2.1.7: altronate dehydratase EC 4.2.1.8: mannonate dehydratase EC 4.2.1.9: dihydroxy-acid dehydratase EC 4.2.1.10: 3-dehydroquinate dehydratase EC 4.2.1.11: phosphopyruvate hydratase (enolase) EC 4.2.1.12: phosphogluconate dehydratase EC 4.2.1.13: Now EC 4.3.1.17, L-serine ammonia-lyase EC 4.2.1.14: Now EC 4.3.1.18, D-serine ammonia-lyase EC 4.2.1.15: Identical with EC 4.4.1.1 cystathionine γ-lyase EC 4.2.1.16: Now EC 4.3.1.19, threonine ammonia-lyase EC 4.2.1.17: enoyl-CoA hydratase EC 4.2.1.18: methylglutaconyl-CoA hydratase EC 4.2.1.19: imidazoleglycerol-phosphate dehydratase EC 4.2.1.20: tryptophan synthase EC 4.2.1.21: Now EC 4.2.1.22 cystathionine β-synthase EC 4.2.1.22: cystathionine β-synthase EC 4.2.1.23: deleted, the reaction was due to a side-reaction of EC 4.2.1.22 cystathionine β-synthase EC 4.2.1.24: porphobilinogen synthase EC 4.2.1.25: L-arabinonate dehydratase EC 4.2.1.26: identical to EC 4.3.1.9, glucosaminate ammonia-lyase EC 4.2.1.27: acetylenecarboxylate hydratase EC 4.2.1.28: propanediol dehydratase EC 4.2.1.29: Now EC 4.99.1.6, indoleacetaldoxime dehydratase EC 4.2.1.30: glycerol dehydratase EC 4.2.1.31: maleate hydratase EC 4.2.1.32: L(+)-tartrate dehydratase EC 4.2.1.33: 3-isopropylmalate dehydratase EC 4.2.1.34: (S)-2-methylmalate dehydratase EC 4.2.1.35: (R)-2-methylmalate dehydratase EC 4.2.1.36: homoaconitate hydratase EC 4.2.1.37: Now EC 3.3.2.4, trans-epoxysuccinate hydrolase EC 4.2.1.38: Now EC 4.3.1.20, erythro-3-hydroxyaspartate ammonia-lyase EC 4.2.1.39: gluconate dehydratase EC 4.2.1.40: glucarate dehydratase EC 4.2.1.41: 5-dehydro-4-deoxyglucarate dehydratase EC 4.2.1.42: galactarate dehydratase EC 4.2.1.43: 2-dehydro-3-deoxy-L-arabinonate dehydratase EC 4.2.1.44: myo-inosose-2 dehydratase EC 4.2.1.45: CDP-glucose 4,6-dehydratase EC 4.2.1.46: dTDP-glucose 4,6-dehydratase EC 4.2.1.47: GDP-mannose 4,6-dehydratase EC 4.2.1.48: D-glutamate cyclase EC 4.2.1.49: urocanate hydratase EC 4.2.1.50: pyrazolylalanine synthase EC 4.2.1.51: prephenate dehydratase EC 4.2.1.52: Now EC 4.3.3.7, 4-hydroxy-2,3,4,5-tetrahydrodipicolinate synthase. EC 4.2.1.53: oleate hydratase EC 4.2.1.54: lactoyl-CoA dehydratase EC 4.2.1.55: 3-hydroxybutyryl-CoA dehydratase EC 4.2.1.56: itaconyl-CoA hydratase EC 4.2.1.57: isohexenylglutaconyl-CoA hydratase EC 4.2.1.58: The reaction described is covered by EC 4.2.1.59 EC 4.2.1.59: 3-hydroxyacyl-[acyl-carrier-protein] dehydratase EC 4.2.1.60: 3-hydroxydecanoyl-(acyl-carrier-protein) dehydratase EC 4.2.1.61: The reaction described is covered by EC 4.2.1.59. EC 4.2.1.62: 5α-hydroxysteroid dehydratase EC 4.2.1.63: Now known to comprise two enzymes, microsomal epoxide hydrolase (EC 3.3.2.9) and soluble epoxide hydrolase (EC 3.3.2.10) EC 4.2.1.64: Now known to comprise two enzymes, microsomal epoxide hydrolase (EC 3.3.2.9) and soluble epoxide hydrolase (EC 3.3.2.10) EC 4.2.1.65: 3-cyanoalanine hydratase EC 4.2.1.66: cyanide hydratase EC 4.2.1.67: D-fuconate dehydratase EC 4.2.1.68: L-fuconate dehydratase EC 4.2.1.69: cyanamide hydratase EC 4.2.1.70: pseudouridylate synthase EC 4.2.1.71: identical to EC 4.2.1.27, acetylenecarboxylate hydratase EC 4.2.1.72: Now EC 4.1.1.78, acetylenedicarboxylate decarboxylase EC 4.2.1.73: protoaphin-aglucone dehydratase (cyclizing) EC 4.2.1.74: long-chain-enoyl-CoA hydratase EC 4.2.1.75: uroporphyrinogen-III synthase EC 4.2.1.76: UDP-glucose 4,6-dehydratase EC 4.2.1.77: trans-L-3-hydroxyproline dehydratase EC 4.2.1.78: Now EC 3.5.99.14, (S)-norcoclaurine synthase EC 4.2.1.79: 2-methylcitrate dehydratase EC 4.2.1.80: 2-oxopent-4-enoate hydratase EC 4.2.1.81: D(-)-tartrate dehydratase EC 4.2.1.82: xylonate dehydratase EC 4.2.1.83: 4-oxalmesaconate hydratase EC 4.2.1.84: nitrile hydratase EC 4.2.1.85: dimethylmaleate hydratase EC 4.2.1.86: identical to EC 4.2.1.98, 16α-hydroxyprogesterone dehydratase EC 4.2.1.87: octopamine dehydratase EC 4.2.1.88: synephrine dehydratase EC 4.2.1.89: The activity has now been shown to be due to EC 2.8.3.21, L-carnitine CoA-transferase and EC 4.2.1.149, crotonobetainyl-CoA hydratase EC 4.2.1.90: L-rhamnonate dehydratase EC 4.2.1.91: arogenate dehydratase EC 4.2.1.92: hydroperoxide dehydratase EC 4.2.1.93: ATP-dependent NAD(P)H-hydrate dehydratase EC 4.2.1.94: scytalone dehydratase EC 4.2.1.95: kievitone hydratase EC 4.2.1.96: 4a-hydroxytetrahydrobiopterin dehydratase EC 4.2.1.97: phaseollidin hydratase EC 4.2.1.98: 16α-hydroxyprogesterone dehydratase EC 4.2.1.99: 2-methylisocitrate dehydratase EC 4.2.1.100: cyclohexa-1,5-dienecarbonyl-CoA hydratase EC 4.2.1.101: Now included with EC 4.1.2.61, feruloyl-CoA hydratase/lyase EC 4.2.1.102: Now EC 4.2.1.100, cyclohexa-1,5-dienecarbonyl-CoA hydratase EC 4.2.1.103: cyclohexyl-isocyanide hydratase EC 4.2.1.104: cyanase EC 4.2.1.105: 2-hydroxyisoflavanone dehydratase EC 4.2.1.106: bile-acid 7α-dehydratase EC 4.2.1.107: 3α,7α,12α-trihydroxy-5β-cholest-24-enoyl-CoA hydratase EC 4.2.1.108: ectoine synthase EC 4.2.1.109: methylthioribulose 1-phosphate dehydratase EC 4.2.1.110: aldos-2-ulose dehydratase EC 4.2.1.111: 1,5-anhydro-D-fructose dehydratase EC 4.2.1.112: acetylene hydratase EC 4.2.1.113: o-succinylbenzoate synthase EC 4.2.1.114: methanogen homoaconitase EC 4.2.1.115: UDP-N-acetylglucosamine 4,6-dehydratase (configuration-inverting) EC 4.2.1.116: 3-hydroxypropionyl-CoA dehydratase EC 4.2.1.117: 2-methylcitrate dehydratase (2-methyl-trans-aconitate forming) EC 4.2.1.118: 3-dehydroshikimate dehydratase EC 4.2.1.119: enoyl-CoA hydratase 2 EC 4.2.1.120: 4-hydroxybutanoyl-CoA dehydratase EC 4.2.1.121: colneleate synthase EC 4.2.1.122: tryptophan synthase (indole-salvaging) EC 4.2.1.123: tetrahymanol synthase EC 4.2.1.124: arabidiol synthase EC 4.2.1.125: dammarenediol II synthase EC 4.2.1.126: N-acetylmuramic acid 6-phosphate etherase EC 4.2.1.127: linalool dehydratase EC 4.2.1.128: lupan-3β,20-diol synthase EC 4.2.1.129: squalene—hopanol cyclase EC 4.2.1.130: D-lactate dehydratase EC 4.2.1.131: carotenoid 1,2-hydratase EC 4.2.1.132: 2-hydroxyhexa-2,4-dienoate hydratase EC 4.2.1.133: copal-8-ol diphosphate hydratase EC 4.2.1.134: very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase EC 4.2.1.135: UDP-N-acetylglucosamine 4,6-dehydratase (configuration-retaining) EC 4.2.1.136: ADP-dependent NAD(P)H-hydrate dehydratase EC 4.2.1.137: sporulenol synthase EC 4.2.1.138: (+)-Caryolan-1-ol synthase EC 4.2.1.139: pterocarpan synthase EC 4.2.1.140: gluconate/galactonate dehydratase EC 4.2.1.141: 2-dehydro-3-deoxyD-arabinonate dehydratase EC 4.2.1.142: 5′-oxoaverantin cyclase EC 4.2.1.143: versicolorin B synthase EC 4.2.1.144: 3-amino-5-hydroxybenzoate synthase EC 4.2.1.145: capreomycidine synthase EC 4.2.1.146: L-galactonate dehydratase EC 4.2.1.147: 5,6,7,8-tetrahydromethanopterin hydro-lyase EC 4.2.1.148: 2-methylfumaryl-CoA hydratase EC 4.2.1.149: crotonobetainyl-CoA hydratase EC 4.2.1.150: short-chain-enoyl-CoA hydratase EC 4.2.1.151: chorismate dehydratase EC 4.2.1.152: hydroperoxy icosatetraenoate dehydratase EC 4.2.1.153: 3-methylfumaryl-CoA hydratase EC 4.2.1.154: tetracenomycin F2 cyclase EC 4.2.1.155: (methylthio)acryloyl-CoA hydratase EC 4.2.1.156: L-talarate dehydratase EC 4.2.1.157: (R)-2-hydroxyisocaproyl-CoA dehydratase EC 4.2.1.158: galactarate dehydratase (D-threo-forming) EC 4.2.1.159: dTDP-4-dehydro-6-deoxy-α-glucopyranose 2,3-dehydratase EC 4.2.1.160: 2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one isomerase/dehydratase EC 4.2.1.161: bisanhydrobacterioruberin hydratase EC 4.2.1.162: 6-deoxy-6-sulfo-D-gluconate dehydratase EC 4.2.1.163: 2-oxo-hept-4-ene-1,7-dioate hydratase EC 4.2.1.164: dTDP-4-dehydro-2,6-dideoxy-D-glucose 3-dehydratase EC 4.2.1.165: chlorophyllide a 31-hydratase EC 4.2.1.166: phosphinomethylmalate isomerase EC 4.2.1.167: (R)-2-hydroxyglutaryl-CoA dehydratase EC 4.2.1.168: GDP-4-dehydro-6-deoxy-α-D-mannose 3-dehydratase EC 4.2.1.169: 3-vinyl bacteriochlorophyllide d 31-hydratase EC 4.2.1.170: 2-(ω-methylthio)alkylmalate dehydratase EC 4.2.1.171: cis-L-3-hydroxyproline dehydratase EC 4.2.1.172: trans-4-hydroxy-L-proline dehydratase EC 4.2.1.173: ent-8α-hydroxylabd-13-en-15-yl diphosphate synthase EC 4.2.1.174: peregrinol diphosphate synthase EC 4.2.1.175: (R)-3-(aryl)lactoyl-CoA dehydratase EC 4.2.1.176: L-lyxonate dehydratase EC 4.2.1.177: (2S)-3-sulfopropanediol dehydratase
EC 4.2.2: Acting on Polysaccharides EC 4.2.2.1: hyaluronate lyase EC 4.2.2.2: pectate lyase EC 4.2.2.3: mannuronate-specific alginate lyase EC 4.2.2.4: Now known to comprise two enzymes: EC 4.2.2.20, chondroitin-sulfate-ABC endolyase and EC 4.2.2.21, chondroitin-sulfate-ABC exolyase EC 4.2.2.5: chondroitin AC lyase EC 4.2.2.6: oligogalacturonide lyase EC 4.2.2.7: heparin lyase EC 4.2.2.8: heparin-sulfate lyase EC 4.2.2.9: pectate disaccharide-lyase EC 4.2.2.10: pectin lyase EC 4.2.2.11: guluronate-specific alginate lyase EC 4.2.2.12: xanthan lyase EC 4.2.2.13: exo-(1→4)-α-D-glucan lyase EC 4.2.2.14: glucuronan lyase EC 4.2.2.15: anhydrosialidase EC 4.2.2.16: levan fructotransferase (DFA-IV-forming) EC 4.2.2.17: inulin fructotransferase (DFA-I-forming) EC 4.2.2.18: inulin fructotransferase (DFA-III-forming) EC 4.2.2.19: chondroitin B lyase EC 4.2.2.20: chondroitin-sulfate-ABC endolyase EC 4.2.2.21: chondroitin-sulfate-ABC exolyase EC 4.2.2.22: pectate trisaccharide-lyase EC 4.2.2.23: rhamnogalacturonan endolyase EC 4.2.2.24: rhamnogalacturonan exolyase EC 4.2.2.25: gellan lyase EC 4.2.2.26: oligo-alginate lyase EC 4.2.2.27: pectin monosaccharide-lyase
EC 4.2.3: Acting on phosphates EC 4.2.3.1: threonine synthase EC 4.2.3.2: ethanolamine-phosphate phospho-lyase EC 4.2.3.3: methylglyoxal synthase EC 4.2.3.4: 3-dehydroquinate synthase EC 4.2.3.5: chorismate synthase EC 4.2.3.6: trichodiene synthase EC 4.2.3.7: pentalenene synthase EC 4.2.3.8: casbene synthase EC 4.2.3.9: aristolochene synthase EC 4.2.3.10: (–)-endo-fenchol synthase EC 4.2.3.11: sabinene-hydrate synthase EC 4.2.3.12: 6-pyruvoyltetrahydropterin synthase EC 4.2.3.13: (+)-δ-cadinene synthase EC 4.2.3.14: Now covered by EC 4.2.3.119, (–)-α-pinene synthase, and EC 4.2.3.120, (–)-β-pinene synthase EC 4.2.3.15: myrcene synthase EC 4.2.3.16: (4S)-limonene synthase EC 4.2.3.17: taxadiene synthase EC 4.2.3.18: abietadiene synthase EC 4.2.3.19: ent-kaurene synthase EC 4.2.3.20: (R)-limonene synthase EC 4.2.3.21: vetispiradiene synthase EC 4.2.3.22: germacradienol synthase EC 4.2.3.23: germacrene-A synthase EC 4.2.3.24: amorpha-4,11-diene synthase EC 4.2.3.25: S-linalool synthase EC 4.2.3.26: R-linalool synthase EC 4.2.3.27: isoprene synthase EC 4.2.3.28: ent-cassa-12,15-diene synthase EC 4.2.3.29: ent-sandaracopimaradiene synthase EC 4.2.3.30: ent-pimara-8(14),15-diene synthase EC 4.2.3.31: ent-pimara-9(11),15-diene synthase EC 4.2.3.32: levopimaradiene synthase EC 4.2.3.33: stemar-13-ene synthase EC 4.2.3.34: stemod-13(17)-ene synthase EC 4.2.3.35: syn-pimara-7,15-diene synthase EC 4.2.3.36: terpentetriene synthase EC 4.2.3.37: epi-isozizaene synthase EC 4.2.3.38: α-bisabolene synthase EC 4.2.3.39: epi-cedrol synthase EC 4.2.3.40: (Z)-γ-bisabolene synthase EC 4.2.3.41: elisabethatriene synthase EC 4.2.3.42: aphidicolan-16β-ol synthase EC 4.2.3.43: fusicocca-2,10(14)-diene synthase EC 4.2.3.44: isopimara-7,15-diene synthase EC 4.2.3.45: phyllocladan-16α-ol synthase EC 4.2.3.46: α-farnesene synthase EC 4.2.3.47: β-farnesene synthase EC 4.2.3.48: (3S,6E)-nerolidol synthase EC 4.2.3.49: (3R,6E)-nerolidol synthase EC 4.2.3.50: (+)-α-santalene synthase [(2Z,6Z)-farnesyl diphosphate cyclizing] EC 4.2.3.51: β-phellandrene synthase (neryl-diphosphate-cyclizing) EC 4.2.3.52: (4S)-β-phellandrene synthase (geranyl-diphosphate-cyclizing) EC 4.2.3.53: (+)-endo-β-bergamotene synthase [(2Z,6Z)-farnesyl diphosphate cyclizing] EC 4.2.3.54: (–)-endo-α-bergamotene synthase [(2Z,6Z)-farnesyl diphosphate cyclizing] EC 4.2.3.55: (S)-β-bisabolene synthase EC 4.2.3.56: γ-humulene synthase EC 4.2.3.57: (–)-β-caryophyllene synthase EC 4.2.3.58: longifolene synthase EC 4.2.3.59: (E)-γ-bisabolene synthase EC 4.2.3.60: germacrene C synthase EC 4.2.3.61: 5-epiaristolochene synthase EC 4.2.3.62: (–)-γ-cadinene synthase [(2Z,6E)-farnesyl diphosphate cyclizing] EC 4.2.3.63: (+)-cubenene synthase EC 4.2.3.64: (+)-epicubenol synthase EC 4.2.3.65: zingiberene synthase EC 4.2.3.66: β-selinene cyclase EC 4.2.3.67: cis-muuroladiene synthase EC 4.2.3.68: β-eudesmol synthase EC 4.2.3.69: (+)-α-barbatene synthase EC 4.2.3.70: patchoulol synthase EC 4.2.3.71: (E,E)-germacrene B synthase EC 4.2.3.72: α-gurjunene synthase EC 4.2.3.73: valencene synthase EC 4.2.3.74: presilphiperfolanol synthase EC 4.2.3.75: (–)-germacrene D synthase EC 4.2.3.76: (+)-δ-selinene synthase EC 4.2.3.77: (+)-germacrene D synthase EC 4.2.3.78: β-chamigrene synthase EC 4.2.3.79: thujopsene synthase EC 4.2.3.80: α-longipinene synthase EC 4.2.3.81: exo-α-bergamotene synthase EC 4.2.3.82: α-santalene synthase EC 4.2.3.83: β-santalene synthase EC 4.2.3.84: 10-epi-γ-eudesmol synthase EC 4.2.3.85: α-eudesmol synthase EC 4.2.3.86: 7-epi-α-selinene synthase EC 4.2.3.87: α-guaiene synthase EC 4.2.3.88: viridiflorene synthase EC 4.2.3.89: (+)-β-caryophyllene synthase EC 4.2.3.90: 5-epi-α-selinene synthase EC 4.2.3.91: cubebol synthase EC 4.2.3.92: (+)-γ-cadinene synthase EC 4.2.3.93: δ-guaiene synthase EC 4.2.3.94: γ-curcumene synthase EC 4.2.3.95: (–)-α-cuprenene synthase EC 4.2.3.96: avermitilol synthase EC 4.2.3.97: (–)-δ-cadinene synthase EC 4.2.3.98: (+)-T-muurolol synthase EC 4.2.3.99: labdatriene synthase EC 4.2.3.100: bicyclogermacrene synthase EC 4.2.3.101: 7-epi-sesquithujene synthase EC 4.2.3.102: sesquithujene synthase EC 4.2.3.103: ent-isokaurene synthase EC 4.2.3.104: α-humulene synthase EC 4.2.3.105: tricyclene synthase EC 4.2.3.106: (E)-β-ocimene synthase EC 4.2.3.107: (+)-car-3-ene synthase EC 4.2.3.108: 1,8-cineole synthase EC 4.2.3.109: (–)-sabinene synthase EC 4.2.3.110: (+)-sabinene synthase EC 4.2.3.111: (–)-α-terpineol synthase EC 4.2.3.112: (+)-α-terpineol synthase EC 4.2.3.113: terpinolene synthase EC 4.2.3.114: γ-terpinene synthase EC 4.2.3.115: α-terpinene synthase EC 4.2.3.116: (+)-camphene synthase EC 4.2.3.117: (–)-camphene synthase EC 4.2.3.118: 2-methylisoborneol synthase EC 4.2.3.119: (-)-α-pinene synthase EC 4.2.3.120: (-)-β-pinene synthase EC 4.2.3.121: (+)-α-pinene synthase EC 4.2.3.122: (+)-β-pinene synthase EC 4.2.3.123: β-sesquiphellandrene synthase EC 4.2.3.124: 2-deoxy-scyllo-inosose synthase EC 4.2.3.125: α-muurolene synthase EC 4.2.3.126: γ-muurolene synthase EC 4.2.3.127: β-copaene synthase EC 4.2.3.128: β-cubebene synthase EC 4.2.3.129: (+)-sativene synthase EC 4.2.3.130: tetraprenyl-β-curcumene synthase EC 4.2.3.131: miltiradiene synthase EC 4.2.3.132: neoabietadiene synthase EC 4.2.3.133: α-copaene synthase EC 4.2.3.134: 5-phosphooxy-L-lysine phospho-lyase EC 4.2.3.135: Δ6-protoilludene synthase EC 4.2.3.136: α-isocomene synthase EC 4.2.3.137: (E)-2-epi-β-caryophyllene synthase EC 4.2.3.138: (+)-epi-α-bisabolol synthase EC 4.2.3.139: valerena-4,7(11)-diene synthase EC 4.2.3.140: cis-abienol synthase EC 4.2.3.141: sclareol synthase EC 4.2.3.142: 7-epizingiberene synthase [(2Z,6Z)-farnesyl diphosphate cyclizing] EC 4.2.3.143: kunzeaol synthase EC 4.2.3.144: geranyllinalool synthase EC 4.2.3.145: ophiobolin F synthase EC 4.2.3.146: cyclooctat-9-en-7-ol synthase EC 4.2.3.147: pimaradiene synthase EC 4.2.3.148: cembrene C synthase EC 4.2.3.149: nephthenol synthase EC 4.2.3.150: cembrene A synthase EC 4.2.3.151: pentamethylcyclopentadecatrienol synthase EC 4.2.3.152: 2-epi-5-epi-valiolone synthase EC 4.2.3.153: (5-formylfuran-3-yl)methyl phosphate synthase EC 4.2.3.154: demethyl-4-deoxygadusol synthase EC 4.2.3.155: 2-epi-valiolone synthase EC 4.2.3.156: hydroxysqualene synthase EC 4.2.3.157: (+)-isoafricanol synthase EC 4.2.3.158: (–)-spiroviolene synthase EC 4.2.3.159: tsukubadiene synthase EC 4.2.3.160: (2S,3R,6S,9S)-(–)-protoillud-7-ene synthase EC 4.2.3.161: (3S)-(+)-asterisca-2(9),6-diene synthase EC 4.2.3.162: (–)-α-amorphene synthase EC 4.2.3.163: (+)-corvol ether B synthase EC 4.2.3.164: (+)-eremophilene synthase EC 4.2.3.165: (1R,4R,5S)-(–)-guaia-6,10(14)-diene synthase EC 4.2.3.166: (+)-(1E,4E,6S,7R)-germacra-1(10),4-dien-6-ol synthase EC 4.2.3.167: dolabella-3,7-dien-18-ol synthase EC 4.2.3.168: dolathalia-3,7,11-triene synthase EC 4.2.3.169: 7-epi-α-eudesmol synthase EC 4.2.3.170: 4-epi-cubebol synthase EC 4.2.3.171: (+)-corvol ether A synthase EC 4.2.3.172: 10-epi-juneol synthase EC 4.2.3.173: τ-cadinol synthase EC 4.2.3.174: (2E,6E)-hedycaryol synthase EC 4.2.3.175: 10-epi-cubebol synthase EC 4.2.3.176: sesterfisherol synthase EC 4.2.3.177: β-thujene synthase EC 4.2.3.178: stellata-2,6,19-triene synthase EC 4.2.3.179: guaia-4,6-diene synthase EC 4.2.3.180: pseudolaratriene synthase EC 4.2.3.181: selina-4(15),7(11)-diene synthase EC 4.2.3.182: pristinol synthase EC 4.2.3.183: nezukol synthase EC 4.2.3.184: 5-hydroxy-α-gurjunene synthase EC 4.2.3.185: ent-atiserene synthase EC 4.2.3.186: ent-13-epi-manoyl oxide synthase EC 4.2.3.187: (2Z,6E)-hedycaryol synthase EC 4.2.3.188: β-geranylfarnesene synthase EC 4.2.3.189: 9,13-epoxylabd-14-ene synthase EC 4.2.3.190: manoyl oxide synthase EC 4.2.3.191: cycloaraneosene synthase EC 4.2.3.192: labda-7,13(16),14-triene synthase EC 4.2.3.193: (12E)-labda-8(17),12,14-triene synthase EC 4.2.3.194: (–)-drimenol synthase EC 4.2.3.195: rhizathalene A synthase EC 4.2.3.196: dolabradiene synthase EC 4.2.3.197: eudesmane-5,11-diol synthase EC 4.2.3.198: α-selinene synthase EC 4.2.3.199: (–)-5-epieremophilene synthase EC 4.2.3.200: β-pinacene synthase EC 4.2.3.201: hydropyrene synthase EC 4.2.3.202: hydropyrenol synthase EC 4.2.3.203: isoelisabethatriene synthase EC 4.2.3.204: valerianol synthase EC 4.2.3.205: sodorifen synthase
EC 4.2.99: Other Carbon-Oxygen Lyases EC 4.2.99.1: Now EC 4.2.2.1, hyaluronate lyase EC 4.2.99.2: Now EC 4.2.3.1, threonine synthase EC 4.2.99.3: Now EC 4.2.2.2, pectate lyase EC 4.2.99.4: Now EC 4.2.2.3, poly(β-D-mannuronate) lyase EC 4.2.99.5: deleted EC 4.2.99.6: Now included with EC 4.2.2.4 (chondroitin ABC lyase) and EC 4.2.2.5 (chondroitin AC lyase) EC 4.2.99.7: Now EC 4.2.3.2, ethanolamine-phosphate phospho-lyase EC 4.2.99.8: Now EC 2.5.1.47, cysteine synthase EC 4.2.99.9: Now EC 2.5.1.48, cystathionine γ-synthase EC 4.2.99.10: Now EC 2.5.1.49 EC 2.5.1.49, O-acetylhomoserine aminocarboxypropyltransferase EC 4.2.99.11: Now EC 4.2.3.3, methylglyoxal synthase EC 4.2.99.12: carboxymethyloxysuccinate lyase EC 4.2.99.13: Now EC 2.5.1.50, zeatin 9-aminocarboxyethyltransferase EC 4.2.99.14: Now EC 2.5.1.51, β-pyrazolylalanine synthase EC 4.2.99.15: Now EC 2.5.1.52, L-mimosine synthase EC 4.2.99.16: Now EC 2.5.1.53, uracilylalanine synthase EC 4.2.99.17: Listed as EC 2.5.1.51, β-pyrazolylalanine synthase EC 4.2.99.18: DNA-(apurinic or apyrimidinic site) lyase EC 4.2.99.19: Now EC 4.4.1.23, 2-hydroxypropyl-CoM lyase EC 4.2.99.20: 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase EC 4.2.99.21: isochorismate lyase EC 4.2.99.22: tuliposide A-converting enzyme EC 4.2.99.23: tuliposide B-converting enzyme EC 4.2.99.24: thebaine synthase
EC 4.3: Carbon-Nitrogen Lyases
EC 4.3.1: Ammonia-Lyases EC 4.3.1.1: aspartate ammonia-lyase EC 4.3.1.2: methylaspartate ammonia-lyase EC 4.3.1.3: histidine ammonia-lyase EC 4.3.1.4: formiminotetrahydrofolate cyclodeaminase EC 4.3.1.5: Now divided into EC 4.3.1.23 (tyrosine ammonia-lyase), EC 4.3.1.24 (phenylalanine ammonia-lyase) and EC 4.3.1.25 (phenylalanine/tyrosine ammonia-lyase) EC 4.3.1.6: β-alanyl-CoA ammonia-lyase EC 4.3.1.7: ethanolamine ammonia-lyase EC 4.3.1.8: Now EC 2.5.1.61, hydroxymethylbilane synthase EC 4.3.1.9: glucosaminate ammonia-lyase EC 4.3.1.10: serine-sulfate ammonia-lyase EC 4.3.1.11: Deleted: inadequately characterized EC 4.3.1.12: ornithine cyclodeaminase EC 4.3.1.13: carbamoyl-serine ammonia-lyase EC 4.3.1.14: 3-aminobutyryl-CoA ammonia-lyase EC 4.3.1.15: diaminopropionate ammonia-lyase EC 4.3.1.16: threo-3-hydroxy-L-aspartate ammonia-lyase EC 4.3.1.17: L-serine ammonia-lyase EC 4.3.1.18: D-serine ammonia-lyase EC 4.3.1.19: threonine ammonia-lyase EC 4.3.1.20: erythro-3-hydroxy-L-aspartate ammonia-lyase EC 4.3.1.21: identical to EC 4.3.1.9, glucosaminate ammonia-lyase EC 4.3.1.22: 3,4-dihydroxyphenylalanine reductive deaminase EC 4.3.1.23: tyrosine ammonia-lyase EC 4.3.1.24: phenylalanine ammonia-lyase EC 4.3.1.25: phenylalanine/tyrosine ammonia-lyase EC 4.3.1.26: Now EC 1.21.3.9, dichlorochromopyrrolate synthase EC 4.3.1.27: threo-3-hydroxy-D-aspartate ammonia-lyase EC 4.3.1.28: L-lysine cyclodeaminase EC 4.3.1.29: D-glucosaminate-6-phosphate ammonia-lyase EC 4.3.1.30: dTDP-4-amino-4,6-dideoxy-D-glucose ammonia-lyase EC 4.3.1.31: L-tryptophan ammonia lyase EC 4.3.1.32: 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase
EC 4.3.2: Amidine-Lyases EC 4.3.2.1: argininosuccinate lyase EC 4.3.2.2: adenylosuccinate lyase EC 4.3.2.3: ureidoglycolate lyase EC 4.3.2.4: purine imidazole-ring cyclase EC 4.3.2.5: peptidylamidoglycolate lyase EC 4.3.2.6: γ-L-glutamyl-butirosin B γ-glutamyl cyclotransferase EC 4.3.2.7: glutathione-specific γ-glutamylcyclotransferase EC 4.3.2.8: γ-glutamylamine cyclotransferase EC 4.3.2.9: γ-glutamylcyclotransferase EC 4.3.2.10: imidazole glycerol-phosphate synthase
EC 4.3.3: Amine-Lyases EC 4.3.3.1: 3-ketovalidoxylamine C-N-lyase EC 4.3.3.2: strictosidine synthase EC 4.3.3.3: deacetylisoipecoside synthase EC 4.3.3.4: deacetylipecoside synthase EC 4.3.3.5: 4′-demethylrebeccamycin synthase EC 4.3.3.6: pyridoxal 5′-phosphate synthase (glutamine hydrolysing) EC 4.3.3.7: 4-hydroxy-tetrahydrodipicolinate synthase
EC 4.3.99: Other Carbon-Nitrogen Lyases EC 4.3.99.1: Now EC 4.2.1.104, cyanate hydratase EC 4.3.99.2: Now EC 7.2.4.1, carboxybiotin decarboxylase EC 4.3.99.3: 7-carboxy-7-deazaguanine synthase EC 4.3.99.4: choline trimethylamine-lyase
EC 4.4: Carbon-Sulfur Lyases
EC 4.4.1: Carbon-sulfur lyases (only sub-subclass identified to date) EC 4.4.1.1: cystathionine γ-lyase EC 4.4.1.2: homocysteine desulfhydrase EC 4.4.1.3: dimethylpropiothetin dethiomethylase EC 4.4.1.4: alliin lyase EC 4.4.1.5: lactoylglutathione lyase EC 4.4.1.6: Now included in EC 4.4.1.13, cysteine-S-conjugate β-lyase EC 4.4.1.7: Now included with EC 2.5.1.18 glutathione transferase EC 4.4.1.8: Now included in EC 4.4.1.13, cysteine-S-conjugate β-lyase EC 4.4.1.9: L-3-cyanoalanine synthase EC 4.4.1.10: cysteine lyase EC 4.4.1.11: methionine γ-lyase EC 4.4.1.12: deleted: activity due to EC 2.3.3.15, sulfoacetaldehyde acetyltransferase EC 4.4.1.13: cysteine-S-conjugate β-lyase EC 4.4.1.14: 1-aminocyclopropane-1-carboxylate synthase EC 4.4.1.15: D-cysteine desulfhydrase EC 4.4.1.16: selenocysteine lyase EC 4.4.1.17: holocytochrome-c synthase EC 4.4.1.18: Now EC 1.8.3.5, prenylcysteine oxidase EC 4.4.1.19: phosphosulfolactate synthase EC 4.4.1.20: leukotriene-C4 synthase EC 4.4.1.21: S-ribosylhomocysteine lyase EC 4.4.1.22: S-(hydroxymethyl)glutathione synthase EC 4.4.1.23: 2-hydroxypropyl-CoM lyase EC 4.4.1.24: (2R)-sulfolactate sulfo-lyase EC 4.4.1.25: L-cysteate sulfo-lyase EC 4.4.1.26: olivetolic acid cyclase EC 4.4.1.27: Now EC 3.13.1.5, carbon disulfide hydrolase EC 4.4.1.42: S-adenosyl-L-methionine lyase
EC 4.5: Carbon-Halide Lyases
EC 4.5.1: Carbon-halide lyases (only sub-subclass identified to date) EC 4.5.1.1: DDT-dehydrochlorinase EC 4.5.1.2: 3-chloro-D-alanine dehydrochlorinase EC 4.5.1.3: dichloromethane dehalogenase EC 4.5.1.4: L-2-amino-4-chloropent-4-enoate dehydrochlorinase EC 4.5.1.5: S-carboxymethylcysteine synthase
EC 4.6: Phosphorus-Oxygen Lyases
EC 4.6.1: Phosphorus-oxygen lyases (only sub-subclass identified to date) EC 4.6.1.1: adenylate cyclase EC 4.6.1.2: guanylate cyclase EC 4.6.1.3: Now EC 4.2.3.4, 3-dehydroquinate synthase EC 4.6.1.4: Now EC 4.2.3.5, chorismate synthase EC 4.6.1.5: Now EC 4.2.3.7, pentalenene synthase EC 4.6.1.6: cytidylate cyclase EC 4.6.1.7: Now EC 4.2.3.8, casbene synthase EC 4.6.1.8: Now EC 4.2.3.10, (-)-endo-fenchol synthase EC 4.6.1.9: Now Now EC 4.2.3.11, sabinene-hydrate synthase EC 4.6.1.10: Now EC 4.2.3.12, 6-pyruvoyltetrahydropterin synthase EC 4.6.1.11: Now EC 4.2.3.13, (+)-δ-cadinene synthase EC 4.6.1.12: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase EC 4.6.1.13: phosphatidylinositol diacylglycerol-lyase EC 4.6.1.14: glycosylphosphatidylinositol diacylglycerol-lyase EC 4.6.1.15: FAD-AMP lyase (cyclizing) EC 4.6.1.16: tRNA-intron lyase EC 4.6.1.17: cyclic pyranopterin monophosphate synthase EC 4.6.1.18: pancreatic ribonuclease EC 4.6.1.19: ribonuclease T2 EC 4.6.1.20: ribonuclease U2 EC 4.6.1.21: Enterobacter ribonuclease EC 4.6.1.22: Bacillus subtilis ribonuclease EC 4.6.1.23: ribotoxin * EC 4.6.1.24: ribonuclease T1 EC 4.6.1.25: bacteriophage T4 restriction endoribonuclease RegB
EC 4.7: Carbon-phosphorus lyases
EC 4.7.1: Carbon-phosphorus lyases (only sub-subclass identified to date) EC 4.7.1.1 α-D ribose 1-methylphosphonate 5-phosphate C-P-lyase *
EC 4.8: Nitrogen-oxygen lyases
EC 4.8.1: Hydro-lyases EC 4.8.1.1: L-piperazate synthase EC 4.8.1.2: aliphatic aldoxime dehydratase EC 4.8.1.3: indoleacetaldoxime dehydratase EC 4.8.1.4: phenylacetaldoxime dehydratase
EC 4.98: ATP-independent chelatases
EC 4.98.1: Forming coordination complexes EC 4.98.1.1: protoporphyrin ferrochelatase
EC 4.99: Other Lyases
EC 4.99.1: Sole sub-subclass for lyases that do not belong in the other subclasses EC 4.99.1.1: Now EC 4.98.1.1 EC 4.99.1.2: alkylmercury lyase EC 4.99.1.3: sirohydrochlorin cobaltochelatase EC 4.99.1.4: sirohydrochlorin ferrochelatase EC 4.99.1.5: Now EC 4.8.1.2, aliphatic aldoxime dehydratase EC 4.99.1.6: Now EC 4.8.1.3, indoleacetaldoxime dehydratase EC 4.99.1.7: Now EC 4.8.1.4, phenylacetaldoxime dehydratase EC 4.99.1.8: heme ligase EC 4.99.1.9:: coproporphyrin ferrochelatase EC 4.99.1.10: magnesium dechelatase EC 4.99.1.11: sirohydrochlorin nickelchelatase EC 4.99.1.12: pyridinium-3,5-bisthiocarboxylic acid mononucleotide nickel chelatase
References