engineering

Lithocholate 6beta-hydroxylase

Lithocholate 6beta-hydroxylase is a engineering topic covered in the lgStudy science library. This page brings together a partial reference excerpt, illustrations, worked examples, real-world applications and a short study plan, so you can understand Lithocholate 6beta-hydroxylase rather than just read about it. In short: In enzymology, a lithocholate 6beta-hydroxylase (EC 1.14.13.94) is an enzyme that catalyzes the chemical reaction lithocholate + NADPH + H+ + O2 ⇌ {\displaystyle \rightleftharpoons } 6beta-hydroxylithocholate + NADP+ + H2O The 4 substrates of this enzyme are lithocholate, NADPH, H+, and O2, whereas its 3 products are 6beta-hydroxylithocholate, NADP+, and H2O. This enzyme belongs to the family of oxidoreductases, spe…

Key takeaways

Lithocholate 6beta-hydroxylase belongs to engineering; place it in that map before memorising details.
Learn the definition first, then one example that makes the definition concrete.
Connect Lithocholate 6beta-hydroxylase to a quantity you can measure, compute or draw — that is where exam questions come from.
Reproduce the core statement of Lithocholate 6beta-hydroxylase from memory before moving on to harder problems.

Reference excerpt

In enzymology, a lithocholate 6beta-hydroxylase (EC 1.14.13.94) is an enzyme that catalyzes the chemical reaction

lithocholate + NADPH + H+ + O2 ⇌ {\displaystyle \rightleftharpoons } 6beta-hydroxylithocholate + NADP+ + H2O The 4 substrates of this enzyme are lithocholate, NADPH, H+, and O2, whereas its 3 products are 6beta-hydroxylithocholate, NADP+, and H2O. This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2 with NADH or NADPH as one donor, and incorporation of one atom o oxygen into the other donor. The systematic name of this enzyme class is lithocholate,NADPH:oxygen oxidoreductase (6beta-hydroxylating). Other names in common use include lithocholate 6beta-monooxygenase, CYP3A10, 6beta-hydroxylase, and cytochrome P450 3A10/lithocholic acid 6beta-hydroxylase.

References

Teixeira J, Gil G (1991). "Cloning, expression, and regulation of lithocholic acid 6 beta-hydroxylase". J. Biol. Chem. 266 (31): 21030–6. doi:10.1016/S0021-9258(18)54816-X. PMID 1840595. Chang TK, Teixeira J, Gil G, Waxman DJ (1993). "The lithocholic acid 6 beta-hydroxylase cytochrome P-450, CYP 3A10, is an active catalyst of steroid-hormone 6 beta-hydroxylation". Biochem. J. 291 (Pt 2): 429–33. doi:10.1042/bj2910429. PMC 1132543. PMID 8484723. Subramanian A, Wang J, Gil G (1998). "STAT 5 and NF-Y are involved in expression and growth hormone-mediated sexually dimorphic regulation of cytochrome P450 3A10/lithocholic acid 6beta-hydroxylase". Nucleic Acids Res. 26 (9): 2173–8. doi:10.1093/nar/26.9.2173. PMC 147524. PMID 9547277. Russell DW (2003). "The enzymes, regulation, and genetics of bile acid synthesis". Annu. Rev. Biochem. 72 (1): 137–74. doi:10.1146/annurev.biochem.72.121801.161712. PMID 12543708.

Worked examples

Example 1 — a first encounter with Lithocholate 6beta-hydroxylase

Start with the simplest possible case. Write down what Lithocholate 6beta-hydroxylase claims or describes in one sentence, then invent the smallest concrete situation in which that sentence is true. In engineering, the smallest case is usually a single object, a single equation or a single measurement. Check that every symbol or term in your sentence has a meaning in that case.

Example 2 — changing one variable

Take the situation from Example 1 and change exactly one quantity: double it, halve it, or set it to zero. Predict what should happen to Lithocholate 6beta-hydroxylase before you calculate. Comparing your prediction with the result is the fastest way to find out whether you understand the idea or only the words.

Example 3 — an exam-style question

Typical questions about Lithocholate 6beta-hydroxylase ask you to (a) state it precisely, (b) apply it to given data, and (c) explain a limitation. Practise writing all three answers in under five minutes; the third part is what separates a full-mark answer from an average one.

Applications of Lithocholate 6beta-hydroxylase

In research: Lithocholate 6beta-hydroxylase appears in engineering research whenever the underlying quantities have to be modelled precisely. Papers usually cite it as a starting assumption and then explore where it breaks down.
In technology and industry: Engineering practice reuses Lithocholate 6beta-hydroxylase in design rules, simulations and safety margins. Knowing the idea lets you read a specification sheet and understand why the numbers look the way they do.
In the classroom: Lithocholate 6beta-hydroxylase is common in secondary-school and first-year university syllabi. It links to neighbouring topics EC 1.14.13, EC 1.14.13 stubs, Enzymes of unknown structure, so understanding it makes those chapters shorter.
In everyday life: Look for Lithocholate 6beta-hydroxylase outside the textbook — in sport, cooking, traffic, electronics or the sky above you. An example you found yourself is remembered far longer than one you were given.

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How to study Lithocholate 6beta-hydroxylase in 20 minutes

Read the reference excerpt below once, without taking notes.
Close the page and write down what Lithocholate 6beta-hydroxylase means in your own words.
Compare your version with the excerpt and mark what you missed.
Work through the three examples above with pen and paper.
Explain Lithocholate 6beta-hydroxylase out loud to somebody else — or to Teacher Smith in the lgStudy chat.

Frequently asked questions

What is Lithocholate 6beta-hydroxylase in simple terms?

In enzymology, a lithocholate 6beta-hydroxylase (EC 1.14.13.94) is an enzyme that catalyzes the chemical reaction lithocholate + NADPH + H+ + O2 ⇌ {\displaystyle \rightleftharpoons } 6beta-hydroxylithocholate + NADP+ + H2O The 4 substrates of this enzyme are lithocholate, NADPH, H+, and O2, whereas…

Why does Lithocholate 6beta-hydroxylase matter?

Because it connects several engineering ideas at once: it gives you a definition you can apply, a quantity you can calculate, and a way to check whether a result is plausible.

How should I study Lithocholate 6beta-hydroxylase?

Read the excerpt, restate it from memory, then work through the examples and applications listed on this page. The five-step study plan above takes about twenty minutes.

What does this page cover?

It gives you a compact reference excerpt plus original lgStudy explanations, examples, applications and study material on Lithocholate 6beta-hydroxylase.