Membrane vesicle trafficking in eukaryotic animal cells involves movement of biochemical signal molecules from synthesis-and-packaging locations in the Golgi body to specific release locations on the inside of the plasma membrane of the secretory cell. It takes place in the form of Golgi membrane-bound micro-sized vesicles, termed membrane vesicles (MVs). In this process, the packed cellular products are released or secreted outside the cell, across its membrane. On the other hand, the vesicular membrane is retained and recycled by the secretory cells. This phenomenon has a major role in synaptic neurotransmission, endocrine secretion, mucous secretion, granular-product secretion by neutrophils, and other phenomena. The scientists behind this discovery were awarded Nobel Prize for the year 2013. In prokaryotic, gram-negative bacterial cells, membrane vesicle trafficking is mediated through bacterial outer membrane bounded nano-sized vesicles, called outer membrane vesicles (OMVs). In this case, however, the OMV membrane is secreted as well, along with OMV-contents to outside the secretion-active bacterium. This different phenomenon has a major role in host–pathogen interactions, endotoxic shock in patients, invasion and infection of animals or plants, inter-species bacterial competition, quorum sensing, exocytosis, and other areas.
Movement within eukaryotic cells
Once vesicles are produced in the endoplasmic reticulum and modified in the Golgi body they make their way to a variety of destinations within the cell. Vesicles first leave the Golgi body and are released into the cytoplasm in a process called budding. Vesicles are then moved towards their destination by motor proteins. Once the vesicle arrives at its destination it joins with the bi-lipid layer in a process called fusion, and then releases its contents.
Budding Receptors embedded in the membrane of the Golgi body bind specific cargo (such as dopamine) on the lumenal side of the vesicle. These cargo receptors then recruit a variety of proteins including other cargo receptors and coat proteins such as clathrin, COPI and COPII. As more and more of these coating proteins come together, they cause the vesicle to bud outward and eventually break free into the cytoplasm. The coating proteins are then shed into the cytoplasm to be recycled and reused.
Motility between cell compartments For movement between different compartments within the cell, vesicles rely on the motor proteins myosin, kinesin (primarily anterograde transport) and dynein (primarily retrograde transport). One end of the motor proteins attaches to the vesicle while the other end attaches to either microtubulees or microfilaments. The motor proteins then move by hydrolyzing ATP, which propels the vesicle towards its destination.
Docking and Fusion As a vesicle nears its intended location, RAB proteins in the vesicle membrane interact with docking proteins at the destination site. These docking proteins bring the vesicle in closer to interact with the SNARE Complex found in the target membrane. The SNARE complex reacts with synaptobrevin found on the vesicle membrane. This forces the vesicle membrane against the membrane of the target complex (or the outer membrane of the cell) and causes the two membranes to fuse. Depending on whether the vesicle fuses with a target complex or the outer membrane, the contents of the vesicle are then released either into the target complex or outside the cell.
Examples in eukaryotes Intracellular trafficking occurs between subcellular compartments like Golgi cisternae and multivesicular endosomes for transport of soluble proteins as MVs. Budding of MVs directly from plasma membrane as microvesicles released outside the secretory cells. Exosomes are MVs that can form inside an internal compartment like multivesicular endosome. Exosomes are released eventually due to fusion of this endosome with plasma membrane of cell. Hijacking of exosomal machinery by some viruses like retroviruses, wherein viruses bud inside multivesicular endosomes and get secreted subsequently as exosomes. All these types (1–4) of modes of membrane vesicle trafficking, taking place in eukaryotic cells have been explained diagrammatically.
In prokaryotes Unlike in eukaryotes, membrane vesicular trafficking in prokaryotes is an emerging area in interactive biology for intra-species (quorum sensing) and inter-species signaling at the host–pathogen interface, as most prokaryotes (except anammox and cyanobacteria) lack internal membrane-compartmentalization of their cytoplasm. Bacterial outer membrane vesicle dispersion along the cell surface was measured in live Escherichia coli, commensal bacteria common in the human gut. Antibiotic treatment altered vesicle dynamics, vesicle-to-membrane affinity, and surface properties of the cell membranes, generally enhancing vesicle transport along the surfaces of bacterial membranes and suggesting that their motion properties could be a signature of antibiotic stress.
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