In enzymology, a mycothiol-dependent formaldehyde dehydrogenase (EC 1.1.1.306) is an enzyme that catalyzes the chemical reaction:
S-(hydroxymethyl)mycothiol + NAD+ ⇌ {\displaystyle \rightleftharpoons } S-formylmycothiol + NADH + + The two substrates of this enzyme are S-(hydroxymethyl)mycothiol and oxidised nicotinamide adenine dinucleotide (NAD+). Its products are S-formylmycothiol, reduced NADH, and a proton. The enzyme belongs to the family of oxidoreductases, specifically those acting on the aldehyde or oxo group of donor with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is formaldehyde:NAD+ oxidoreductase (mycothiol-formylating). This enzyme is also called NAD/factor-dependent formaldehyde dehydrogenase or S-(hydroxymethyl)mycothiol dehydrogenase.
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