chemistry

N-acyl-D-amino-acid deacylase

N-acyl-D-amino-acid deacylase is a chemistry topic covered in the lgStudy science library. This page brings together a partial reference excerpt, illustrations, worked examples, real-world applications and a short study plan, so you can understand N-acyl-D-amino-acid deacylase rather than just read about it. In short: In enzymology, a N-acyl-D-amino-acid deacylase (EC 3.5.1.81) is an enzyme that catalyzes the chemical reaction N-acyl-D-amino acid + H2O ⇌ {\displaystyle \rightleftharpoons } an acid + D-amino acid Thus, the two substrates of this enzyme are N-acyl-D-amino acid and H2O, whereas its two products are acid and D-amino acid. This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other tha…

Key takeaways

N-acyl-D-amino-acid deacylase belongs to chemistry; place it in that map before memorising details.
Learn the definition first, then one example that makes the definition concrete.
Connect N-acyl-D-amino-acid deacylase to a quantity you can measure, compute or draw — that is where exam questions come from.
Reproduce the core statement of N-acyl-D-amino-acid deacylase from memory before moving on to harder problems.

Reference excerpt

In enzymology, a N-acyl-D-amino-acid deacylase (EC 3.5.1.81) is an enzyme that catalyzes the chemical reaction

N-acyl-D-amino acid + H2O ⇌ {\displaystyle \rightleftharpoons } an acid + D-amino acid Thus, the two substrates of this enzyme are N-acyl-D-amino acid and H2O, whereas its two products are acid and D-amino acid. This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amides. The systematic name of this enzyme class is N-acyl-D-amino acid amidohydrolase. It employs one cofactor, zinc.

Structural studies As of late 2007, 8 structures have been solved for this class of enzymes, with PDB accession codes PDB: 1M7J, PDB: 1RJP, PDB: 1RJQ, PDB: 1RJR, PDB: 1RK5, PDB: 1RK6, PDB: 1V4Y, and PDB: 1V51.

References

Wakayama M, Katsuno Y, Hayashi S, Miyamoto Y, Sakai K, Moriguchi M (1995). "Cloning and sequencing of a gene encoding D-aminoacylase from Alcaligenes xylosoxydans subsp. xylosoxydans A-6 and expression of the gene in Escherichia coli". Biosci. Biotechnol. Biochem. 59 (11): 2115–9. doi:10.1271/bbb.59.2115. PMID 8541651. Wakayama M, Hayashi S, Yatsuda Y, Katsuno Y, Sakai K, Moriguchi M (1996). "Overproduction of D-aminoacylase from Alcaligenes xylosoxydans subsp. xylosoxydans A-6 in Escherichia coli and its purification". Protein Expr. Purif. 7 (4): 395–9. doi:10.1006/prep.1996.0059. PMID 8776758.

Worked examples

Example 1 — a first encounter with N-acyl-D-amino-acid deacylase

Start with the simplest possible case. Write down what N-acyl-D-amino-acid deacylase claims or describes in one sentence, then invent the smallest concrete situation in which that sentence is true. In chemistry, the smallest case is usually a single object, a single equation or a single measurement. Check that every symbol or term in your sentence has a meaning in that case.

Example 2 — changing one variable

Take the situation from Example 1 and change exactly one quantity: double it, halve it, or set it to zero. Predict what should happen to N-acyl-D-amino-acid deacylase before you calculate. Comparing your prediction with the result is the fastest way to find out whether you understand the idea or only the words.

Example 3 — an exam-style question

Typical questions about N-acyl-D-amino-acid deacylase ask you to (a) state it precisely, (b) apply it to given data, and (c) explain a limitation. Practise writing all three answers in under five minutes; the third part is what separates a full-mark answer from an average one.

Applications of N-acyl-D-amino-acid deacylase

In research: N-acyl-D-amino-acid deacylase appears in chemistry research whenever the underlying quantities have to be modelled precisely. Papers usually cite it as a starting assumption and then explore where it breaks down.
In technology and industry: Engineering practice reuses N-acyl-D-amino-acid deacylase in design rules, simulations and safety margins. Knowing the idea lets you read a specification sheet and understand why the numbers look the way they do.
In the classroom: N-acyl-D-amino-acid deacylase is common in secondary-school and first-year university syllabi. It links to neighbouring topics EC 3.5.1, EC 3.5 stubs, Enzymes of known structure, so understanding it makes those chapters shorter.
In everyday life: Look for N-acyl-D-amino-acid deacylase outside the textbook — in sport, cooking, traffic, electronics or the sky above you. An example you found yourself is remembered far longer than one you were given.

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How to study N-acyl-D-amino-acid deacylase in 20 minutes

Read the reference excerpt below once, without taking notes.
Close the page and write down what N-acyl-D-amino-acid deacylase means in your own words.
Compare your version with the excerpt and mark what you missed.
Work through the three examples above with pen and paper.
Explain N-acyl-D-amino-acid deacylase out loud to somebody else — or to Teacher Smith in the lgStudy chat.

Frequently asked questions

What is N-acyl-D-amino-acid deacylase in simple terms?

In enzymology, a N-acyl-D-amino-acid deacylase (EC 3.5.1.81) is an enzyme that catalyzes the chemical reaction N-acyl-D-amino acid + H2O ⇌ {\displaystyle \rightleftharpoons } an acid + D-amino acid Thus, the two substrates of this enzyme are N-acyl-D-amino acid and H2O, whereas its two products are…

Why does N-acyl-D-amino-acid deacylase matter?

Because it connects several chemistry ideas at once: it gives you a definition you can apply, a quantity you can calculate, and a way to check whether a result is plausible.

How should I study N-acyl-D-amino-acid deacylase?

Read the excerpt, restate it from memory, then work through the examples and applications listed on this page. The five-step study plan above takes about twenty minutes.

What does this page cover?

It gives you a compact reference excerpt plus original lgStudy explanations, examples, applications and study material on N-acyl-D-amino-acid deacylase.