Carbohydrate NMR spectroscopy is the application of nuclear magnetic resonance (NMR) spectroscopy to structural and conformational analysis of carbohydrates. This method allows the scientists to elucidate structure of monosaccharides, oligosaccharides, polysaccharides, glycoconjugates and other carbohydrate derivatives from synthetic and natural sources. Among structural properties that could be determined by NMR are primary structure (including stereochemistry), saccharide conformation, stoichiometry of substituents, and ratio of individual saccharides in a mixture. Modern high field NMR instruments used for carbohydrate samples, typically 500 MHz or higher, are able to run a suite of 1D, 2D, and 3D experiments to determine a structure of carbohydrate compounds.
Carbohydrate NMR observables
Chemical shift
Common chemical shift ranges for nuclei within carbohydrate residues are:
Typical 1H NMR chemical shifts of carbohydrate ring protons are 3–6 ppm (4.5–5.5 ppm for anomeric protons). Typical 13C NMR chemical shifts of carbohydrate ring carbons are 60–110 ppm In the case of simple mono- and oligosaccharide molecules, all proton signals are typically separated from one another (usually at 500 MHz or better NMR instruments) and can be assigned using 1D NMR spectrum only. However, bigger molecules exhibit significant proton signal overlap, especially in the non-anomeric region (3-4 ppm). Carbon-13 NMR overcomes this disadvantage by larger range of chemical shifts and special techniques allowing to block carbon-proton spin coupling, thus making all carbon signals high and narrow singlets distinguishable from each other. The typical ranges of specific carbohydrate carbon chemical shifts in the unsubstituted monosaccharides are:
Anomeric carbons: 90-100 ppm Sugar ring carbons bearing a hydroxy function: 68-77 Open-form sugar carbons bearing a hydroxy function: 71-75 Sugar ring carbons bearing an amino function: 50-56 Exocyclic hydroxymethyl groups: 60-64 Exocyclic carboxy groups: 172-176 Desoxygenated sugar ring carbons: 31-40 A carbon at pyranose ring closure: 71-73 (α-anomers), 74-76 (β-anomers) A carbon at furanose ring closure: 80-83 (α-anomers), 83-86 (β-anomers)
Coupling constants
Direct carbon-proton coupling constants are used to study the anomeric configuration of a sugar. Vicinal proton-proton coupling constants are used to study stereo orientation of protons relatively to the other protons within a sugar ring, thus identifying a monosaccharide. Vicinal heteronuclear H-C-O-C coupling constants are used to study torsional angles along glycosidic bond between sugars or along exocyclic fragments, thus revealing a molecular conformation. Sugar rings are relatively rigid molecular fragments, thus vicinal proton-proton couplings are characteristic:
Equatorial to axial: 1–4 Hz Equatorial to equatorial: 0–2 Hz Axial to axial non-anomeric: 9–11 Hz Axial to axial anomeric: 7–9 Hz Axial to exocyclic hydroxymethyl: 5 Hz, 2 Hz Geminal between hydroxymethyl protons: 12 Hz
Nuclear Overhauser effects (NOEs)
NOEs are sensitive to interatomic distances, allowing their usage as a conformational probe, or proof of a glycoside bond formation. It's a common practice to compare calculated to experimental proton-proton NOEs in oligosaccharides to confirm a theoretical conformational map. Calculation of NOEs implies an optimization of molecular geometry.
Other NMR observables Relaxivities, nuclear relaxation rates, line shape and other parameters were reported useful in structural studies of carbohydrates.
Elucidation of carbohydrate structure by NMR spectroscopy
Structural parameters of carbohydrates The following is a list of structural features that can be elucidated by NMR:
Chemical structure of each carbohydrate residue in a molecule, including carbon skeleton size and sugar type (aldose/ketose) cycle size (pyranose/furanose/linear) stereo configuration of all carbons (monosaccharide identification) stereo configuration of anomeric carbon (α/β) absolute configuration (D/L) location of amino-, carboxy-, deoxy- and other functions Chemical structure of non-carbohydrate residues in molecule (amino acids, fatty acids, alcohols, organic aglycons etc.) Substitution positions in residues Sequence of residues Stoichiometry of terminal residues and side chains Location of phosphate and sulfate diester bonds Polymerization degree and frame positioning (for polysaccharides)
NMR spectroscopy vs. other methods Widely known methods of structural investigation, such as mass-spectrometry and X-ray analysis are only limitedly applicable to carbohydrates. Such structural studies, such as sequence determination or identification of new monosaccharides, benefit the most from the NMR spectroscopy. Absolute configuration and polymerization degree are not always determinable using NMR only, so the process of structural elucidation may require additional methods. Although monomeric composition can be solved by NMR, chromatographic and mass-spectroscopic methods provide this information sometimes easier. The other structural features listed above can be determined solely by the NMR spectroscopic methods. The limitation of the NMR structural studies of carbohydrates is that structure elucidation can hardly be automatized and require a human expert to derive a structure from NMR spectra.
Application of various NMR techniques to carbohydrates Complex glycans possess a multitude of overlapping signals, especially in a proton spectrum. Therefore, it is advantageous to utilize 2D experiments for the assignment of signals. The table and figures below list most widespread NMR techniques used in carbohydrate studies.
Research scheme NMR spectroscopic research includes the following steps:
Extraction of carbohydrate material (for natural glycans) Chemical removal of moieties masking regularity (for polymers) Separation and purification of carbohydrate material (for 2D NMR experiments, 10 mg or more is recommended) Sample preparation (usually in D2O) Acquisition of 1D spectra Planning, acquisition and processing of other NMR experiments (usually requires from 5 to 20 hours) Assignment and interpretation of spectra (see exemplary figure) If a structural problem could not be solved: chemical modification/degradation and NMR analysis of products Acquisition of spectra of the native (unmasked) compound and their interpretation based on modified structure Presentation of results
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