Nucleic acid methods are the techniques used to study nucleic acids: DNA and RNA.
Purification DNA extraction Phenol–chloroform extraction Minicolumn purification RNA extraction Boom method Synchronous coefficient of drag alteration (SCODA) DNA purification
Quantification Abundance in weight: spectroscopic nucleic acid quantitation Absolute abundance in number: real-time polymerase chain reaction (quantitative PCR) High-throughput relative abundance: DNA microarray High-throughput absolute abundance: serial analysis of gene expression (SAGE) Size: gel electrophoresis
Synthesis De novo: oligonucleotide synthesis Amplification: polymerase chain reaction (PCR), loop-mediated isothermal amplification (LAMP), transcription-mediated amplification (TMA)
Kinetics Multi-parametric surface plasmon resonance Dual-polarization interferometry Quartz crystal microbalance with dissipation monitoring (QCM-D)
Gene function RNA interference
Other Bisulfite sequencing DNA sequencing Expression cloning Fluorescence in situ hybridization Lab-on-a-chip Comparison of nucleic acid simulation software Northern blot Nuclear run-on assay Radioactivity in the life sciences Southern blot Differential centrifugation (sucrose gradient) Toeprinting assay Several bioinformatics methods, as seen in list of RNA structure prediction software
See also CSH Protocols Current Protocols
References
External links Protocols for Recombinant DNA Isolation, Cloning, and Sequencing