PRR29

Key takeaways

PRR29 belongs to biology; place it in that map before memorising details.
Learn the definition first, then one example that makes the definition concrete.
Connect PRR29 to a quantity you can measure, compute or draw — that is where exam questions come from.
Reproduce the core statement of PRR29 from memory before moving on to harder problems.

Reference excerpt

PRR29 (proline-rich protein 29) is a protein encoded by the PRR29 gene located in humans on chromosome 17 at 17q23. Its function is not fully understood. Its name is derived from the chain of 5 proline amino acids located toward the end of the protein. The primary domain within the sequence of this protein is known as DUF4587. It is reported to have high levels of expression in tissues pertaining to the circulatory system and the immune system. It is hypothesized that PRR29 is a nuclear protein that facilitates communication between the nucleus and the mitochondria. The gene is also commonly known as C17orf72. The gene has a size of 5961 base pairs and contains five exons.

Gene

PRR29 is located on the long arm of chromosome 17 (17q23.3), starting at 63998344 and ending at 64004305. The gene spans 5961 base pairs and is oriented on the plus strand. Genes SNHG25 and LOC105371858 neighbor PRR29 on chromosome 17.The gene ICAM2 is located on the negative strand, directly opposite of PRR29. The human gene for PRR29, also referred to as C17of72, spans 6 exons and is located at the genomic coordinates chr17:63,998,351-64,002,516 on the positive DNA strand (hg38). It is 4,166 base pairs in length including introns. After they have been removed, the length is shortened to 3,611 base pairs.

mRNA The gene has 12 common splice variants and one unspliced form. The longest transcribed mRNA is made up of 3048 base pairs and the transcribed protein sequence for this mRNA is 189 amino acids.

Protein

General properties Homo sapiens PRR29 has several protein isoforms, with the longest being 236 amino acids. PRR29 has a predicted Isoelectric point of 5.23 and a predicted Molecular weight of 26.1 kilodaltons. PRR29 is characterized by a larger than average proportion of prolines (19.1%) and a smaller than average amount of asparagines (0.4%) The PRR29 protein is estimated to have a molecular weight of 20.7 kDa. Its isoelectric point is predicted to lie at 4.83.

Domains PRR29 contains a proline rich region within its sequence from amino acids 73 to 166. A domain of unknown function, DUF 4587, is also present from amino acids 39 to 112 or 113. DUF 4587 is usually between 64 and 79 amino acids long and contains the two sequence motifs QNAQ and HHH. PRR29 is predicted to contain multiple alpha helix and beta-sheet forming regions. Specifically, the DUF 4587 region is predicted to form an alpha helix. It contains one proline-rich region motif that extends from amino acid 39 to 107.

Secondary and Tertiary Structure

The secondary structure is characterized by high confidence in the presence of an alpha helix from amino acid 43 to 70 with the rest consisting of coils. In terms of tertiary structure, predictive tools returned low confidence in all sections of the protein besides the core alpha helix.

Untranslated Regions The secondary structure of the 5' UTR of PRR29 consists of a singular stem-loop that is almost wholly conserved between orthologs. The 3' UTR is much longer, containing 41 different stem loops in its secondary structure. Any predicted miRNA binding to PRR29 is believed to be nonfunctional.

Subcellular localization Using PSORTII, PRR29 is predicted to localize in the nucleus of the cell. PSORTII does not predict any targeting sequences or signal peptides. Data on localization of PRR29 within cells shows that it is primarily found in the nucleus, followed by the mitochondria and cytoplasm.

Post-Translational Modification PRR29 is predicted to undergo sumoylation, acetylation, and serine, threonine and tyrosine phosphorylation. The most common types of post-translational modification that occur for this protein are phosphorylation, glycosylation, hydroxylation, and sumoylation. They contribute to the stability, structure, and folding of the protein.

Interactions The interactome of PRR29 is not yet well characterized. One experimental study found that a Sus scrofa PRR29-like protein interacts with the N-terminal protease of classical swine fever virus (CSFV).

Expression

PRR29 is ubiquitously expressed throughout the body. However, there is particularly high expression in the ovaries, muscle, heart, testes, and thymus. According to PaxDb, PRR29 abundance falls in the bottom 5% relative to other proteins. Its expression is concentrated in related tissues including the spleen, lungs, white blood cells, and heart. In situ hybridization data reveals that expression in the human brain is brain is relatively low in the forebrain but higher in the basal ganglia, midbrain, and hindbrain with the exception of the cerebellar cortex. For comparison, expression in the mouse brain is focused in the isocortex, olfactory region, hippocampus, cortical subplate, and cerebellum.

Transcript-Level Regulation Prediction of transcription factors that bind to the promoter region for PRR29 include ones involved with the activation of leukocytes and the formation of blood cells. Abundance of the protein relative to others found in the human body is currently unknown.

Homology

PRR29 has a single known paralog, C21orf58. PRR29 is well conserved among chordates and PRR29-like proteins containing the DUF 4587 have been predicted in protostomes, such as Mollusca and Annelida. DUF 4587 is highly conserved in all PRR29 orthologs and is also present in its paralog, C21orf58. This domain has been found in species as distantly related as Capitella teleta, which diverged from humans 847 million years ago. This gene has orthologs in other animal species. It has only been found in vertebrates with the oldest being the whitespotted bamboo shark. PRR29 does not have any known paralogs.

Evolution

This gene evolves at a rate higher than that of cytochrome c but lower than that of fibrinogen alpha chain.

Clinical Significance It is unknown if PRR29 is directly linked to any diseases. Experiments have been performed on tissue and cell samples in order to observe any potential changes in expression of the protein under different conditions. Samples inflicted with pulmonary sarcoidosis and small cell lung cancer did not experience and significant changes in expression compared to normal cells. Intracranial artery aneurysm did induce change, leading to heightened expression

References

External links Human Expression Atlas data (EBI) Genecards

Illustrations

Worked examples

Example 1 — a first encounter with PRR29

Start with the simplest possible case. Write down what PRR29 claims or describes in one sentence, then invent the smallest concrete situation in which that sentence is true. In biology, the smallest case is usually a single object, a single equation or a single measurement. Check that every symbol or term in your sentence has a meaning in that case.

Example 2 — changing one variable

Take the situation from Example 1 and change exactly one quantity: double it, halve it, or set it to zero. Predict what should happen to PRR29 before you calculate. Comparing your prediction with the result is the fastest way to find out whether you understand the idea or only the words.

Example 3 — an exam-style question

Typical questions about PRR29 ask you to (a) state it precisely, (b) apply it to given data, and (c) explain a limitation. Practise writing all three answers in under five minutes; the third part is what separates a full-mark answer from an average one.

Applications of PRR29

In research: PRR29 appears in biology research whenever the underlying quantities have to be modelled precisely. Papers usually cite it as a starting assumption and then explore where it breaks down.
In technology and industry: Engineering practice reuses PRR29 in design rules, simulations and safety margins. Knowing the idea lets you read a specification sheet and understand why the numbers look the way they do.
In the classroom: PRR29 is common in secondary-school and first-year university syllabi. It links to neighbouring topics Genes on human chromosome 17, Proteins, so understanding it makes those chapters shorter.
In everyday life: Look for PRR29 outside the textbook — in sport, cooking, traffic, electronics or the sky above you. An example you found yourself is remembered far longer than one you were given.

How to study PRR29 in 20 minutes

Read the reference excerpt below once, without taking notes.
Close the page and write down what PRR29 means in your own words.
Compare your version with the excerpt and mark what you missed.
Work through the three examples above with pen and paper.
Explain PRR29 out loud to somebody else — or to Teacher Smith in the lgStudy chat.

Frequently asked questions

What is PRR29 in simple terms?

PRR29 (proline-rich protein 29) is a protein encoded by the PRR29 gene located in humans on chromosome 17 at 17q23. Its function is not fully understood.

Why does PRR29 matter?

Because it connects several biology ideas at once: it gives you a definition you can apply, a quantity you can calculate, and a way to check whether a result is plausible.

How should I study PRR29?

Read the excerpt, restate it from memory, then work through the examples and applications listed on this page. The five-step study plan above takes about twenty minutes.

What does this page cover?

It gives you a compact reference excerpt plus original lgStudy explanations, examples, applications and study material on PRR29.