engineering

Peptide-O-fucosyltransferase

Peptide-O-fucosyltransferase is a engineering topic covered in the lgStudy science library. This page brings together a partial reference excerpt, illustrations, worked examples, real-world applications and a short study plan, so you can understand Peptide-O-fucosyltransferase rather than just read about it. In short: In enzymology, a peptide-O-fucosyltransferase (EC 2.4.1.221) is an enzyme that catalyzes the chemical reaction in which an alpha-L-fucosylpyranoside residue is transferred from GDP-beta-L-fucose to the sidechain oxygen atom of a serine or threonine residue in a protein. This enzyme belongs to the family of glycosyltransferases, specifically the hexosyltransferases.

Key takeaways

Peptide-O-fucosyltransferase belongs to engineering; place it in that map before memorising details.
Learn the definition first, then one example that makes the definition concrete.
Connect Peptide-O-fucosyltransferase to a quantity you can measure, compute or draw — that is where exam questions come from.
Reproduce the core statement of Peptide-O-fucosyltransferase from memory before moving on to harder problems.

Reference excerpt

In enzymology, a peptide-O-fucosyltransferase (EC 2.4.1.221) is an enzyme that catalyzes the chemical reaction in which an alpha-L-fucosylpyranoside residue is transferred from GDP-beta-L-fucose to the sidechain oxygen atom of a serine or threonine residue in a protein. This enzyme belongs to the family of glycosyltransferases, specifically the hexosyltransferases. The systematic name of this enzyme class is GDP-beta-L-fucose:polypeptide O-alpha-L-fucosyltransferase. Other names in common use include GDP-L-fucose:polypeptide fucosyltransferase, GDP-fucose protein O-fucosyltransferase, and GDP-fucose:polypeptide fucosyltransferase.

References

Wang Y, Spellman MW (1998). "Purification and characterization of a GDP-fucose:polypeptide fucosyltransferase from Chinese hamster ovary cells". J. Biol. Chem. 273 (14): 8112–8. doi:10.1074/jbc.273.14.8112. PMID 9525914. Haltiwanger RS; Shao, L; Shi, S; Harris, RJ; Spellman, MW; Stanley, P; Haltiwanger, RS (2001). "Modification of epidermal growth factor-like repeats with O-fucose Molecular cloning and expression of a novel GDP-fucose protein O-fucosyltransferase". J. Biol. Chem. 276 (43): 40338–45. doi:10.1074/jbc.M107849200. PMID 11524432. Wang Y, Lee GF, Kelley RF, Spellman MW (1996). "Identification of a GDP-L-fucose:polypeptide fucosyltransferase and enzymatic addition of O-linked fucose to EGF domains". Glycobiology. 6 (8): 837–42. doi:10.1093/glycob/6.8.837. PMID 9023546. Peter-Katalinic J; Huwiler, KG; Macek, B; Hess, D; Lawler, J; Mosher, DF; Peter-Katalinic, J (2001). "C-mannosylation and O-fucosylation of the thrombospondin type 1 module". J. Biol. Chem. 276 (9): 6485–98. doi:10.1074/jbc.M008073200. PMID 11067851.

Worked examples

Example 1 — a first encounter with Peptide-O-fucosyltransferase

Start with the simplest possible case. Write down what Peptide-O-fucosyltransferase claims or describes in one sentence, then invent the smallest concrete situation in which that sentence is true. In engineering, the smallest case is usually a single object, a single equation or a single measurement. Check that every symbol or term in your sentence has a meaning in that case.

Example 2 — changing one variable

Take the situation from Example 1 and change exactly one quantity: double it, halve it, or set it to zero. Predict what should happen to Peptide-O-fucosyltransferase before you calculate. Comparing your prediction with the result is the fastest way to find out whether you understand the idea or only the words.

Example 3 — an exam-style question

Typical questions about Peptide-O-fucosyltransferase ask you to (a) state it precisely, (b) apply it to given data, and (c) explain a limitation. Practise writing all three answers in under five minutes; the third part is what separates a full-mark answer from an average one.

Applications of Peptide-O-fucosyltransferase

In research: Peptide-O-fucosyltransferase appears in engineering research whenever the underlying quantities have to be modelled precisely. Papers usually cite it as a starting assumption and then explore where it breaks down.
In technology and industry: Engineering practice reuses Peptide-O-fucosyltransferase in design rules, simulations and safety margins. Knowing the idea lets you read a specification sheet and understand why the numbers look the way they do.
In the classroom: Peptide-O-fucosyltransferase is common in secondary-school and first-year university syllabi. It links to neighbouring topics EC 2.4.1, EC 2.4 stubs, Enzymes of unknown structure, so understanding it makes those chapters shorter.
In everyday life: Look for Peptide-O-fucosyltransferase outside the textbook — in sport, cooking, traffic, electronics or the sky above you. An example you found yourself is remembered far longer than one you were given.

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How to study Peptide-O-fucosyltransferase in 20 minutes

Read the reference excerpt below once, without taking notes.
Close the page and write down what Peptide-O-fucosyltransferase means in your own words.
Compare your version with the excerpt and mark what you missed.
Work through the three examples above with pen and paper.
Explain Peptide-O-fucosyltransferase out loud to somebody else — or to Teacher Smith in the lgStudy chat.

Frequently asked questions

What is Peptide-O-fucosyltransferase in simple terms?

Why does Peptide-O-fucosyltransferase matter?

Because it connects several engineering ideas at once: it gives you a definition you can apply, a quantity you can calculate, and a way to check whether a result is plausible.

How should I study Peptide-O-fucosyltransferase?

Read the excerpt, restate it from memory, then work through the examples and applications listed on this page. The five-step study plan above takes about twenty minutes.

What does this page cover?

It gives you a compact reference excerpt plus original lgStudy explanations, examples, applications and study material on Peptide-O-fucosyltransferase.