A protist () is any eukaryotic organism (one with cells containing a nucleus) that is not an animal, plant, or fungus. The protists do not form a natural group, or clade, since they exclude certain eukaryotes with whom they share a common ancestor; but, like algae or invertebrates, the grouping is used for convenience. In some systems of biological classification, such as the five-kingdom scheme proposed by Robert Whittaker in 1969, the protists make up a kingdom called Protista, composed of "organisms which are unicellular or unicellular-colonial and which form no tissues". In the 21st century, the classification shifted toward a phylogenetic classification of protists. The following groups contain protists. The clade Opisthokonta also contains the animals and the fungi, and the Archaeplastida also contains plants.
Cavalier-Smith's classification Thomas Cavalier-Smith and his collaborators use a fully rank-based classification of protists. Here is their classification:
Current classification Legend:
† Extinct, or exclusively fossil taxon. (P) Paraphyletic or polyphyletic taxon. (P?) Potentially paraphyletic or polyphyletic taxon. (=...) Taxonomic synonym. (...) Same taxon in a different code of nomenclature.
Amorphea The supergroup Amorphea contains very diverse heterotrophic organisms, from the macroscopic fungi and animals to the unicellular choanoflagellates and classical amoebae. They frequently exhibit the ability to produce multinucleated cells, a trait considered ancestral to Amorphea. They are also capable of producing pseudopodia, as does the closely related CRuMs clade, forming the clade Podiata. Amorphea is divided into two clades: Amoebozoa, containing well-known amoebae and slime molds, and Obazoa, containing animals, fungi, and their closest relatives. The relationship between these two clades was initially called 'Unikonta', due to a hypothesis where their common ancestor was a unikont, i.e., a eukaryote with just one flagellum. However, this hypothesis was refuted, as there are bikont amorpheans (e.g., Breviata anathema) and it likely is not an ancestral trait to have a single flagellum.
Obazoa
The clade Obazoa contains two small groups of flagellates, the breviates and the apusomonads, and the large clade Opisthokonta, which contains animals, fungi, and their closest protist relatives. Under the Cavalier-Smith system, breviates and apusomonads were two classes that composed the phylum Apusozoa, but this taxon is paraphyletic, as apusomonads are more closely related to opisthokonts. The taxonomy of apusomonads was expanded in a 2022 phylogenetic study that introduced many new genera.
Class Breviatea Cavalier-Smith 2004, order Breviatida Cavalier-Smith 2004. Genus not assigned to any family: Lenisia. Family Breviatidae Cavalier-Smith 2013, genera Breviata, Subulatomonas. Family Pygsuidae Cavalier-Smith 2022, genus Pygsuia. Class Thecomonadea Cavalier-Smith 1993 emend. 2013, order Apusomonadida Karpov & Mylnikov 1989, family Apusomonadidae Karpov & Mylnikov 1989. Genera not assigned to any subfamily: Amastigomonas, Multimonas, Podomonas, Catacumbia, Cavaliersmithia, Karpovia, Singekia. Subfamily Apusomonadinae Cavalier-Smith 2010, genera Apusomonas (=Rostromonas), Manchomonas. Subfamily Thecamonadinae Larsen & Patterson 1990, genera Chelonemonas, Thecamonas.
Opisthokonta Opisthokonts are divided into two branches: Holozoa (animal-related), containing the ichthyosporeans, pluriformeans, filastereans and choanoflagellates; and Nucletmycea or Holomycota (fungus-related), containing the nucleariids and opisthosporidians. These groups, minus opisthosporidians, were classified as different classes within the paraphyletic phylum Choanozoa in the system of Cavalier-Smith, now obsolete. Instead, Choanozoa is the name used for the clade containing choanoflagellates and animals. Opisthosporidians (aphelids, rozellids and microsporidians) are often studied as protists, but are also considered fungi by protistologists and mycologists alike. Both Holozoa and Nucletmycea have been proposed once as superkingdoms by a group of mycologists who classified nucleariids and fungi as kingdoms, but without any mention of holozoan kingdoms.
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