Seed testing

Key takeaways

Seed testing belongs to science; place it in that map before memorising details.
Learn the definition first, then one example that makes the definition concrete.
Connect Seed testing to a quantity you can measure, compute or draw — that is where exam questions come from.
Reproduce the core statement of Seed testing from memory before moving on to harder problems.

Reference excerpt

Seed testing is an analysis of seeds for viability. Seed testing performed for a number of reasons, including research purposes or to determine if seed storage techniques are functioning. Common seed tests include Germination tests, Viability tests, purity tests and weed tests. The first two are common for scientific research. For commercially sold seed, all four of these tests are done in dedicated laboratories by trained and usually certified analysts. The tests are designed to evaluate the quality of the seed lot being sold.

Germination tests A germination test reports the percentage of seed that germinated. In commercial settings, tests are usually made in either 200 or 400 seed samples.

Viability test The Tetrazolium Chloride (TZ) test, often called the quick germination test, is a chemical test used to determine seed viability, and results are usually available within 24 to 48 hours The TZ test differs from a germination test in that the TZ test can give you an early and quick snapshot of seed viability but is not a replacement for the more comprehensive seed germination test. In Canada, the TZ test is not officially recognized by the CFIA (except for western wheatgrass where the TZ result may be added to the germination for a final germination total). In the United States, the TZ test can be used as a replacement for a germination test, although a follow-up germination test is usually recommended.

Advantages of the TZ test A rapid evaluation of seed viability. Detect seed weaknesses before they become evident in germination tests. Timely guidance in quality control programs. Properly conducted TZ and germination test results are generally in close agreement and within the range of normal sampling variation. Differences of 3% to 5% may be due to an unavoidable sampling variation error. Differences between TZ and germination test results are usually smaller in high quality seed than in low quality seed, with large seeded crops than with small-seeded crops, and with uniform seed lots than with non-uniform lots.

Disadvantages of the TZ test Requires specialized training and experience. Test is usually more laborious and tedious to perform than a germination test. Test results do not reflect fungal infection or chemical damage. Test results do not reflect dormancy. This method is invasive, meaning that the seeds, used in a testing, are dissected and cannot be used for germination.

Procedure A viability test (TZ test) is a test for viability that involves the steps: preconditioning (imbibition) and then preparation and staining (sometimes cutting the seed and then soaking the seed in a 2,3,5 triphenyl tetrazolium chloride solution). Seeds are soaked in water overnight. They may be pre-moistened, in which case the seeds are allowed to imbibe water between a moistened germination paper blotter. Seeds are then dissected, either longitudinally or transversely, with a scalpel so that the embryo is exposed to the tetrazolium chloride solution. One half of this seed is used for the test and the other half is discarded. Staining is done with a solution of 2, 3, 5 triphyenyl tetrazolium chloride (a salt) is added to water to form a colorless solution. The seeds are placed in a 1% solution (for legumes and grasses that are not bisected), or a dilute 0.1% solution for bisected grasses and cereals. Seed coats of legumes must usually be removed or peeled back before examination. Care must be taken to prevent breaking of radicles and other damage to the seeds.

Evaluation Dehydrogenase enzymes present in living tissue reduce the tetrazolium chloride to formazan, a reddish, water-insoluble compound. This reaction occurs in or near living cells, which are releasing hydrogen in respiration processes. Depending on size, all seeds are examined under a microscope at 10-30 power. Larger seeds, such as peas, may be examined without a microscope. Analysts look for three things: Sound tissues, weak living tissues or dead tissues. Sound tissues produce a normal red color and resist the penetration of tetrazolium. The rate of hydrogen released in sound tissue is slow in comparison to that in partially weakened tissues. Weak living tissues produce an abnormal color. These tissues have lost some of their initial resistance to the penetration of tetrazolium. Respiration is accelerated and formazan is produced rapidly. During the early stages of deterioration, these tissues become darker red (bruised) quicker than sound, healthy tissues. Dead tissues do not stain, remaining usually white (aged tissue) because the lack of respiration prevents the production of formazan. TZ test results are recorded as a percentage and are usually reported in the remarks section of the Certificate of Seed Analysis issued by the seed laboratory. Hard seeds are to be reported (if applicable) as a percentage and are included in the total percent viable seed. Regardless of how they are reported, TZ test results for viability give you an estimate of the maximum percentage of seeds that have the potential to produce normal seedlings.

Purity test A purity test reports the percentage of seed described on the label that is actually found in the quantity of seed.

Weed test A weed test examines a sample of seed and identifies every seed that is different from the labeled seed kind.

References

External links Association of Official Seed Certifying Agencies Society of Commercial Seed Technologists Association of American Seed Control Officials (AASCO) Association of Official Seed Analysts

Illustrations

Worked examples

Example 1 — a first encounter with Seed testing

Start with the simplest possible case. Write down what Seed testing claims or describes in one sentence, then invent the smallest concrete situation in which that sentence is true. In science, the smallest case is usually a single object, a single equation or a single measurement. Check that every symbol or term in your sentence has a meaning in that case.

Example 2 — changing one variable

Take the situation from Example 1 and change exactly one quantity: double it, halve it, or set it to zero. Predict what should happen to Seed testing before you calculate. Comparing your prediction with the result is the fastest way to find out whether you understand the idea or only the words.

Example 3 — an exam-style question

Typical questions about Seed testing ask you to (a) state it precisely, (b) apply it to given data, and (c) explain a limitation. Practise writing all three answers in under five minutes; the third part is what separates a full-mark answer from an average one.

Applications of Seed testing

In research: Seed testing appears in science research whenever the underlying quantities have to be modelled precisely. Papers usually cite it as a starting assumption and then explore where it breaks down.
In technology and industry: Engineering practice reuses Seed testing in design rules, simulations and safety margins. Knowing the idea lets you read a specification sheet and understand why the numbers look the way they do.
In the classroom: Seed testing is common in secondary-school and first-year university syllabi. It links to neighbouring topics Seeds, so understanding it makes those chapters shorter.
In everyday life: Look for Seed testing outside the textbook — in sport, cooking, traffic, electronics or the sky above you. An example you found yourself is remembered far longer than one you were given.

How to study Seed testing in 20 minutes

Read the reference excerpt below once, without taking notes.
Close the page and write down what Seed testing means in your own words.
Compare your version with the excerpt and mark what you missed.
Work through the three examples above with pen and paper.
Explain Seed testing out loud to somebody else — or to Teacher Smith in the lgStudy chat.

Frequently asked questions

What is Seed testing in simple terms?

Seed testing is an analysis of seeds for viability. Seed testing performed for a number of reasons, including research purposes or to determine if seed storage techniques are functioning.

Why does Seed testing matter?

Because it connects several science ideas at once: it gives you a definition you can apply, a quantity you can calculate, and a way to check whether a result is plausible.

How should I study Seed testing?

Read the excerpt, restate it from memory, then work through the examples and applications listed on this page. The five-step study plan above takes about twenty minutes.

What does this page cover?

It gives you a compact reference excerpt plus original lgStudy explanations, examples, applications and study material on Seed testing.