biology

Sulfate permease

Sulfate permease is a biology topic covered in the lgStudy science library. This page brings together a partial reference excerpt, illustrations, worked examples, real-world applications and a short study plan, so you can understand Sulfate permease rather than just read about it. In short: The sulfate permease (SulP) family (TC# 2.A.53) is a member of the large APC superfamily of secondary carriers. The SulP family is a large and ubiquitous family of proteins derived from archaea, bacteria, fungi, plants and animals.

Key takeaways

Sulfate permease belongs to biology; place it in that map before memorising details.
Learn the definition first, then one example that makes the definition concrete.
Connect Sulfate permease to a quantity you can measure, compute or draw — that is where exam questions come from.
Reproduce the core statement of Sulfate permease from memory before moving on to harder problems.

Reference excerpt

The sulfate permease (SulP) family (TC# 2.A.53) is a member of the large APC superfamily of secondary carriers. The SulP family is a large and ubiquitous family of proteins derived from archaea, bacteria, fungi, plants and animals. Many organisms including Bacillus subtilis, Synechocystis sp, Saccharomyces cerevisiae, Arabidopsis thaliana and Caenorhabditis elegans possess multiple SulP family paralogues. Many of these proteins are functionally characterized, and most are inorganic anion uptake transporters or anion:anion exchange transporters. Some transport their substrate(s) with high affinities, while others transport it or them with relatively low affinities. Others may catalyze SO2−4:HCO−3 exchange, or more generally, anion:anion antiport. For example, the mouse homologue, SLC26A6 (TC# 2.A.53.2.7), can transport sulfate, formate, oxalate, chloride and bicarbonate, exchanging any one of these anions for another. A cyanobacterial homologue can transport nitrate. Some members can function as channels. SLC26A3 (2.A.53.2.3) and SLC26A6 (2.A.53.2.7 and 2.A.53.2.8) can function as carriers or channels, depending on the transported anion. In these porters, mutating a glutamate, also involved in transport in the CIC family (TC# 2.A.49), (E357A in SLC26A6) created a channel out of the carrier. It also changed the stoichiometry from 2Cl−/HCO−3 to 1Cl−/HCO−3.

Structure All SulPs are homodimers. where two subunits do not function independently. The dimeric structure probably represents the native state of SulP transporters. A low-resolution structure of a bacterial SulP transporter revealed a dimeric stoichiometry, stabilized via its transmembrane core and mobile intracellular domains. The cytoplasmic STAS domain projects away from the transmembrane domain and is not involved in dimerization. The structure suggests that large movements of the STAS domain underlie the conformational changes that occur during transport. The bacterial proteins vary in size from 434 residues to 573 residues with only a few exceptions. The eukaryotic proteins vary in size from 611 residues to 893 residues with a few exceptions. Thus, the eukaryotic proteins are usually larger than the prokaryotic homologues. These proteins exhibit 10-13 putative transmembrane α-helical spanners (TMSs) depending on the protein.

Crystal structures Several crystal structures are available for members of the SulP family through RCSB:

PDB: 4DGF, 4DGH, 3LLO, 2KLN

Homologues One of the distant SulP homologues has been shown to be a bicarbonate:Na+ symporter (TC# 2.A.53.5.1). Bioinformatic work has identified additional homologues with fused domains. Some of these fused proteins have SulP homologues fused to carbonic anhydrase homologues (TC# 2.A.53.8.1). These are also presumed to be bicarbonate uptake permeases. Another has SulP fused to Rhodanese, a sulfate:cyanide sulfotransferase (TC# 2.A.53.9.1). This SulP homologue is presumably a sulfate transporter. Homologues currently characterized in the SulP family can be found in the Transporter Classification Database.

SLC26A3 in mice One member of the SulP family, SLC26A3, has been knocked out in mice. Apical membrane chloride/base exchange activity was sharply reduced, and the luminal content was more acidic in SLC26A3-null mouse colon. The epithelial cells in the colon displayed unique adaptive regulation of ion transporters; NHE3 expression was enhanced in the proximal and distal colon, whereas colonic H+/K+-ATPase and the epithelial sodium channel showed massive up-regulation in the distal colon. Plasma aldosterone was increased in SLC26A3-null mice. Thus, SLC26A3 may be the major apical chloride/base exchanger and is essential for the absorption of chloride in the colon. In addition, SLC26A3 regulates colonic crypt proliferation. Deletion of SLC26A3 results in chloride-rich diarrhea and is associated with compensatory adaptive up-regulation of ion-absorbing transporters.

MOT1 MOT1 from Arabidopsis thaliana (TC# 2.A.53.11.1, 456aas; 8-10 TMSs), a distant homologue of the SulP and BenE (2.A.46) families, is expressed in both roots and shoots, and is localized to plasma membranes and intracellular vesicles. MOT1 is required for efficient uptake and translocation of molybdate as well as for normal growth under conditions of limited molybdate supply. Kinetic studies in yeast revealed that the K(m) value of MOT1 for molybdate is approximately 20 nM. Mo uptake by MOT1 in yeast is not affected by the presence of sulfate. MOT1 did not complement a sulfate transporter-deficient yeast mutant strain. MOT1 is thus probably specific for molybdate. The high affinity of MOT1 allows plants to obtain scarce Mo from soil when its concentration is about 10nM.

SLC26 SLC26 proteins function as anion exchangers and Cl− channels. Ousingsawat et al. (2012) examined the functional interaction between CF transmembrane conductance regulator (CFTR) and SLC26A9 in polarized airway epithelial cells and in non-polarized HEK293 cells expressing CFTR and SLC26A9 (2.A.56.2.10). They found that SLC26A9 provides a constitutively active basal Cl− conductance in polarized grown CFTR-expressing CFBE airway epithelial cells, but not in cells expressing F508del-CFTR. In polarized CFTR-expressing cells, SLC26A9 also contributes to both Ca2+- and CFTR-activated Cl− secretion. In contrast in non-polarized HEK293 cells co-expressing CFTR/SLC26A9, the baseline Cl− conductance provided by SLC26A9 was inhibited during activation of CFTR. Thus, SLC26A9 and CFTR behave differentially in polarized and non-polarized cells, explaining earlier conflicting data.

Transport Reaction The generalized transport reactions catalyzed by SulP family proteins are:

(1) SO2−4 (out) + nH+ (out) → SO2−4 (in) + nH+ (in). (2) SO2−4 (out) + nHCO−3 (in) ⇌ SO2−4 (in) + nHCO−3 (out). (3) I− and other anions (out) ⇌ I− and other anions (in). (4) HCO−3 (out) + nH+ (out) → HCO−3 (in) + nH+ (in).

See also Solute carrier family Transporter Classification Database Membrane transport protein

References

As of 2 February 2016, this article is derived in whole or in part from Transporter Classification Database. The copyright holder has licensed the content in a manner that permits reuse under CC BY-SA 3.0 and GFDL. All relevant terms must be followed. The original text was at "2.A.53 The Sulfate Permease (SulP) Family"

Worked examples

Example 1 — a first encounter with Sulfate permease

Start with the simplest possible case. Write down what Sulfate permease claims or describes in one sentence, then invent the smallest concrete situation in which that sentence is true. In biology, the smallest case is usually a single object, a single equation or a single measurement. Check that every symbol or term in your sentence has a meaning in that case.

Example 2 — changing one variable

Take the situation from Example 1 and change exactly one quantity: double it, halve it, or set it to zero. Predict what should happen to Sulfate permease before you calculate. Comparing your prediction with the result is the fastest way to find out whether you understand the idea or only the words.

Example 3 — an exam-style question

Typical questions about Sulfate permease ask you to (a) state it precisely, (b) apply it to given data, and (c) explain a limitation. Practise writing all three answers in under five minutes; the third part is what separates a full-mark answer from an average one.

Applications of Sulfate permease

In research: Sulfate permease appears in biology research whenever the underlying quantities have to be modelled precisely. Papers usually cite it as a starting assumption and then explore where it breaks down.
In technology and industry: Engineering practice reuses Sulfate permease in design rules, simulations and safety margins. Knowing the idea lets you read a specification sheet and understand why the numbers look the way they do.
In the classroom: Sulfate permease is common in secondary-school and first-year university syllabi. It links to neighbouring topics Integral membrane proteins, Protein families, Transmembrane transporters, so understanding it makes those chapters shorter.
In everyday life: Look for Sulfate permease outside the textbook — in sport, cooking, traffic, electronics or the sky above you. An example you found yourself is remembered far longer than one you were given.

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How to study Sulfate permease in 20 minutes

Read the reference excerpt below once, without taking notes.
Close the page and write down what Sulfate permease means in your own words.
Compare your version with the excerpt and mark what you missed.
Work through the three examples above with pen and paper.
Explain Sulfate permease out loud to somebody else — or to Teacher Smith in the lgStudy chat.

Frequently asked questions

What is Sulfate permease in simple terms?

Why does Sulfate permease matter?

Because it connects several biology ideas at once: it gives you a definition you can apply, a quantity you can calculate, and a way to check whether a result is plausible.

How should I study Sulfate permease?

Read the excerpt, restate it from memory, then work through the examples and applications listed on this page. The five-step study plan above takes about twenty minutes.

What does this page cover?

It gives you a compact reference excerpt plus original lgStudy explanations, examples, applications and study material on Sulfate permease.